三氧化二砷对涎腺导管癌抑制作用的初步观察  

Preliminary observation of arsenic trioxide inhibitory effect on salivary duct carcinoma

作　　者：吕智勇[1] 王太和[1] 朱广智 杨春艳[1] 

机构地区：[1]哈尔滨医科大学附属第二医院口腔科,哈尔滨150086 [2]内蒙古牙克石市人民医院

出　　处：《实用肿瘤学杂志》2013年第6期534-538,共5页Practical Oncology Journal

摘　　要：目的观察三氧化二砷（As2O3）对人涎腺导管癌（Salivaryductcarcinoma）细胞的抑制作用，探索治疗人涎腺导管细胞癌的新途径。方法建立人涎腺导管癌细胞系，应用不同浓度As2O3在不同时间点作用人涎腺导管癌细胞株，以MTT和形态学分析法观察细胞的变化。结果不同浓度三氧化二砷在不同时间点对人涎腺导管癌细胞均有明娃抑制作用，并呈时间剂量依赖关系，癌细胞异形性逐渐消失，细胞凋亡现象明显，可见凋亡小体。结论As2O3对人涎腺导管癌细胞株具有显著的抑制增殖、诱导分化和促进凋亡作用。Objective To investigate the inhibitory effects of arsenic trioxide on salivary duct carcinoma cell colony and to explore a new approach to treat salivary duct carcinoma. Methods Human salivary duct carci- noma cancer cells were established. The effects of different concentrations （0.5,1,2,4 μmol/L） and time （48,96 H） of arsenic trioxid on salivary duct carcinoma cell colony and the changes of the cell colony were observed with MTT assay and microscopic cell analysis. Results Human salivary duct carcinoma were significantly inhibited by Different concentrations of arsenic trioxide at different times points, and the relationship between the doses and time points are dependent, cell atypia disappearing apoptosis appears apoptotic bodies. Conclusion Arsenic trioxide can significantly inhibit proliferation, induce differentiation and promote apoptosis in the salivary duct carcinoma cells.

关 键 词：三氧化二砷 人涎腺导管癌 细胞凋亡 

分 类 号：R739.87[医药卫生—肿瘤]