针刺对高脂血症小鼠血脂的影响及其机制研究  

Effects of Acupuncture on Blood Lipids in Hyperlipidemia Mice

作　　者：郭佳[1] 姬晓兰[1] 彭博[2] 李富运[2] 张莉[2] 刘承[1] 霍则军[1] 

机构地区：[1]北京大学第三医院,北京市100191 [2]北京中医药大学,北京市100029

出　　处：《中国康复理论与实践》2013年第12期1120-1123,共4页Chinese Journal of Rehabilitation Theory and Practice

基　　金：国家自然科学基金(No.81273837)

摘　　要：目的观察针刺对高脂血症小鼠血脂的影响,探讨针刺逆转内皮功能障碍的机制。方法 40只ApoE(-/-)小鼠随机分为对照组、针刺非穴位组、针刺穴位组和他汀类药物组,分别针刺穴位、非穴位以及辛伐他汀灌胃。8周后测量各组小鼠总胆固醇(TC)。采用ELISA法检测血浆中血管紧张素Ⅱ(AngⅡ)、内皮素-1(ET-1)及一氧化氮(NO)的含量。采用Western blotting法检测小鼠心脏AngⅡ1型受体(AT1R)、内皮素-1A受体(ETAR)的蛋白含量。结果针刺穴位组和他汀类药物组与对照组相比,TC含量降低,血浆AngⅡ、ET-1含量降低,NO含量升高,心脏组织中AT1R、ETAR蛋白含量降低(P<0.05)。结论针刺内关和丰隆穴可以抑制ApoE(-/-)小鼠血脂升高,降低外周血AngⅡ、ET-1水平,升高外周血NO含量,抑制心脏组织中AT1R、ETAR。Objective To observe the effects of acupuncture on blood lipids of hyperlipidemia mice, and explore the mechanism of acu- puncture against endothelial dysfunction. Methods 40 ApoE （-/-） mice were randomly divided into control group （C）, acupuncture at non-acupoint group （D）, acupuncture at acupoint group （E）, and simvastatin group （F） with 10 cases in each group. After 8 weeks, total cho- lesterol （TC）, the plasma angiotensin II （Ang II）, endothelin-1 （ET-1） and nitric oxide （NO） were measured with enzyme-linked immuno sot- bent assay （ELISA）. The mice myocardial angiotensin II type 1 receptor （ATI R）, and endothelin-1 type A receptor （ETAR） protein were de- tected with Western blotting. Results The blood lipid, the content of plasma Ang II and ET-1, and the level of ATIR and ETAR in the heart tissue were significantly lower, and the content of NO was significantly higher in groups E and F than in group C （P〈0.05）. Conclusion Acu- puncture can inhibit the level of blood lipids in ApoE（-/-） mice, reduce the levels ofAng II and ET-1, increase the level of NO in peripheral blood, and inhibit the expression ofAT1R, ETAR in heart tissue.

关 键 词：高脂血症 针刺 血管紧张素Ⅱ 内皮素-1 受体 小鼠 

分 类 号：R224.2[医药卫生—中医基础理论]