氮对纤维藻生长、油脂积累及营养吸收的影响  

Effect of nitrogen on the growth,lipid accumulation and nutrient uptake of a freshwater microalgae Ankistrodsemus sp.

作　　者：张森[1] 刘平怀[1] 王丽波[1] 杨勋[1] 郝宗娣[1] 张玲[1] 罗宁[1] 

机构地区：[1]海南大学材料与化工学院,热带作物种质资源保护与开发利用教育部重点实验室,海南海口570228

出　　处：《食品工业科技》2014年第1期155-159,共5页Science and Technology of Food Industry

基　　金：国家科技支撑计划(2011BAD14B01);海南省中药现代化科技专项(ZY201327);基础研究重大项目前期研究专项(2010CB134409);海南大学植物学国家级重点学科(071001)资助

摘　　要：为研究氮源及氮浓度对一株淡水富油纤维藻（Ankistrodsemussp．）生长、油脂积累及营养吸收的影响。以3N—BBM为基础培养基，采用单因素实验分别考察了尿素、氯化铵、硝酸钠及亚硝酸钠以及硝酸钠浓度对纤维藻生长、油脂积累及营养吸收的影响。结果表明：四种氮源对纤维藻生长和生物量影响较小，但对油脂积累影响较大。该藻在尿素中的生物量浓度最高（2．87g／L），在硝酸钠中的油脂含量（30．17％）和油脂产量（0．81g／L）最高。硝酸钠浓度为0．3mm01／L时，该藻较难存活，浓度为3．0mm01／L时其生长较差，但油脂含量（37．37％）和油脂产量（0．89g/L）最高；浓度在9．0～30．0mmoI／L时，纤维藻生物量较高，但油脂含量和油脂产量较低。综合生物量和油脂含量考虑，该纤维藻最适生长和油脂积累的氮源为硝酸钠且最适浓度为3．0mmo]／L。In order to study effects of nitrogen sources and concentrations on the growth, lipid accumulation, and nutrient uptake of a freshwater microalga Ankistrodsemus sp., four nitrogen sources such as urea, ammonium chloride,sodium nitrate and sodium nitrite and different nitrogen concentrations were investigated based on 3 N-BBM media.The results showed that the growth and biomass were less affected by four nitrogen sources, but it was just opposite for lipid accumulation.The highest biomass（2.87g/L）was obtained with urea as nitrogen source, while the optimal lipid content （30.]7%）and lipid productivity （0.81g/L）was gained with sodium nitrate. Ankistrodsemus sp. was grown poorly and nearly died just on the fourth day with 0.3mmol/L sodium nitrate. Although the growth and biomass was poor with 3mmol/L sodium nitrate, the lipid content （37.37%）and lipid productivity（O.89g/L） was highest.With higher nitrogen concentration（9.0~30.Ommol/L）,Ankistrodsemus sp.was grown vigorously and had higher biomass, but the lipid content and lipid productivity were lower.So sodium nitrate was the optimal nitrogen source for growth and lipid accumulation of Ankistrodsemus sp. and the optimal concentration was 3.0mmol/L

关 键 词：纤维藻 氮 细胞生长 油脂积累 

分 类 号：Q935[生物学—微生物学]