高效脱硫菌的筛选及其性能研究  

Screening of high efficient desulphurization bacteria and study on its properties

作　　者：张秀霞 孔甜甜 陈水泉 王志伟 邵珺 刘欣梅 

机构地区：[1]中国石油大学环境与安全工程系,青岛266580

出　　处：《环境工程学报》2014年第1期378-384,共7页Chinese Journal of Environmental Engineering

基　　金：中央高校基本科研业务费专项基金资助项目(10CX05010A);青岛市科技计划资助项目(11-2-4-4-(12)-jch)

摘　　要：从高硫污染的活性污泥中富集培养,分离纯化得到一株可以降解噻吩的菌株S-4,并对该菌株的形态特征进行观察。应用Design-Expert8.0.5b软件进行响应面优化实验,研究了反应时间、噻吩浓度、微生物浓度3个因素的组合对菌株S-4脱硫效果的影响,并拟合得到多元二次回归方程,得出最佳实验条件。拟合结果表明,当反应时间27.46 h,噻吩浓度为1.04%,微生物浓度4.04%时,预测噻吩降解率为14.8%,通过验证得最佳条件下的降解率为14.3%,与预测值相符。A strain of bacterium named S-4 which can degradate thiophene was enrichment cultured and se- lected from high sulfur polluted activated sludge, and the morphological characteristics of the strain were observed as well. The software Design-Expert 8.0.5b was applied to the response surface optimization experiment. And the influences of three factors which were reaction time, concentration of thiophene and concentration of microor- ganism were studied in the process of desulphurization bacterium S-4 degradation. The optimum experimental conditions and an equation were achieved by the software. The optimum reaction conditions were determined as follows： reaction time of 27.46 h, thiophene concentration of 1.04% , microorganism concentration of 4.04%. Under these conditions, the maximum theoretic thiophene degradation rate was 14.8%. After verifications, the maximum theoretic value was consistent with mean value of verification test, which was 14.3%.

关 键 词：生物脱硫 噻吩 响应面设计 

分 类 号：X703[环境科学与工程—环境工程]