实验性结肠炎大鼠结肠上皮细胞凋亡与肠道通透性的研究  

Study on the relationship between colonic epithelial cell apoptosis and intestinal permeability in rats with experimental colitis

作　　者：袁柏思[1] 周淑萍[2] 路又可[1] 汪芳裕[1] 

机构地区：[1]南京军区南京总医院,江苏南京210002 [2]安徽省淮南市第一人民医院,安徽淮南232007

出　　处：《现代中西医结合杂志》2014年第2期115-116,119,共3页Modern Journal of Integrated Traditional Chinese and Western Medicine

基　　金：国家自然科学基金资助项目(81070289)

摘　　要：目的探讨右旋葡聚硫酸钠(DSS)溶液诱导建立的溃疡性结肠炎(UC)大鼠模型肠上皮细胞凋亡改变与肠黏膜屏障的关系。方法将18只健康SD大鼠随机分成2组:UC组12只和NC组6只,UC组大鼠自由饮用DSS溶液(浓度为40 g/L)7 d,建立急性UC模型,NC组正常饮水,第8天处死大鼠,留取结肠标本,HE染色观察结肠黏膜病变,TUNEL细胞凋亡检测试剂盒检测UC大鼠结肠上皮细胞凋亡数。结果 TUNEL细胞凋亡检测结果显示,UC组大鼠结肠上皮细胞凋亡数量明显高于NC组,细胞凋亡数目和肠黏膜损伤的程度呈正相关。结论 DSS诱导的UC大鼠肠上皮细胞凋亡增加,相邻细胞间的间隙增大,导致肠黏膜屏障受损,从而使肠道通透性增高。Objective It is to explore the relationship between intestinal epithelial cell apoptosis and the intestinal mucosal barrier in rats with DSS -induced experimental ulcerative colitis（UC）. Methods Eighteen Sprague-Dawley rats were assigned randomly to UC group （ n = 12） and normal control （NC） group （ n = 6）. Rats in UC group were fed with 4% DSS for 7 days to establish acute UC model. Rats in NC group were fed with tap water. And then the rats were anesthetized and sacrificed on the eighth day, and the colon samples were collected. Colonic mucosal lesions were observed by HE staining, and the number of apoptotie cells was detected by TUNEL situ cell death detection kit in the rats with DSS - induced colonic epithelium. Re-sults The results of in situ cell death detection kit - POD for detecting the number of apoptotic cells showed that the number of epithelial cell apoptosis was significantly higher in the UC group than that of the normal control group, and there was a positive correlation between the number of apoptotic and intestinal mucosal damage level. Conclusion In the DSS - induced UC rats, the intestinal epithelial cell apoptosis increased and its adjacent cell gap widen which caused damage of intestinal mucosal bar-rier, thus lead to the increasing of intestinal permeability.

关 键 词：溃疡性结肠炎 肠黏膜屏障 通透性 凋亡 

分 类 号：R-332[医药卫生]