植物花色苷对血小板凋亡通路中BCL2家族影响  

Effects of anthocyanin on BCL2 family of platelet apoptosis pathway

作　　者：陈礼仪[1] 田金举 任婧[1] 邓秀娟[1] 陈曦曌 谢瑾[1] 丁焱[1] 杨燕[1] 

机构地区：[1]中山大学公共卫生学院营养系广东省营养膳食与健康重点实验室,广东广州510080 [2]中山大学中山医学院

出　　处：《中国公共卫生》2014年第1期67-69,共3页Chinese Journal of Public Health

基　　金：国家自然科学基金(81372978);国家大学生创新项目(201210558086);2013年广东省医学科学技术研究基金;高校基本科研业务费中山大学;青年教师培育项目(12ykpy12);中山大学实验室开放基金(KF201213);中山大学医科2012年第一批学生业务科研立项项目

摘　　要：目的探讨花色苷矢车菊素-3-葡萄糖苷(Cy-3-g)对血小板凋亡通路中BCL2家族影响。方法采集健康志愿者外周静脉血,制备纯化血小板悬液;纯化后的血小板与终浓度为0.5、5、50μmol/L花色苷Cy-3-g孵育,胶原作为凋亡诱导剂诱导血小板凋亡后,采用流式细胞仪测定血小板凋亡率,提取总蛋白用western blot检测抗凋亡蛋白BCL-XL,促凋亡蛋白BAK、BAX、BID及cleaved BID蛋白表达量。结果与对照组比较,5μmol/L花色苷能够明显增加血小板凋亡率;与对照组比较,5、50μmol/L花色苷组BCL-XL蛋白表达水平[分别为(0.739±0.047)、(0.884±0.004)]明显下调,差异均有统计学意义(P<0.01);与对照组比较,5、50μmol/L花色苷组BAK、BAX、BID蛋白表达水平[分别为BAK:(1.667±0.063)、(1.531±0.039),BAX:(2.271±0.165)、(1.895±0.018),BID:(1.861±0.231)、(1.311±0.121)]明显上调,差异均有统计学意义(P<0.05)。结论花色苷能够通过调控BCL2家族蛋白的表达水平促进血小板凋亡。Objective To investigate the effects of anthocyanin cyanidin-3-glucoside （Cy-3-g） on BCL2 family of platelet apoptosis pathway. Methods Peripheral venous blood samples of healthy volunteers were collected and purified platelet suspension was prepared. Gel-filtered platelets were pre-incubated with 0. 5,5, and 50 ixmol/L Cy-3-g and in duced by collagen. Flow cytometry was used to measure platelet apoptosis rate and total platelet proteins were extracted; the expressions of antiapoptotic proteins BCL-XL and pro-apoptotic proteins BAK, BAX, BID and cleaved BID were quantified with western blot. Results Compared with the control group,5 μmol/L Cy-3-g significantly increased the rate of platelet apoptosis and 5 μmol/L （0. 739 ± 0. 047 ） ,50 p, moVL（0. 884 ± 0. 004）Cy-3-g effectively reduced expression of anti-apoptotic protein BCL-XL in platelets （P 〈 0. 01 ）. Compared with the control group ,5,50 txmol/L Cy-3-g signifi cantly promoted the expressions of proapoptotic proteins BAK, BAX, BID and the differences were of statistic signifi cance at concentrations of 5 p, mol/L（ 1. 667 ± 0. 063,2. 271± 0. 165,1. 861 ±0. 231 ） and 50 ixmol/L （ 1. 531 ± 0. 039, 1.895 ± 0. 018,1.311 ± 0. 121 ）, respectively （ all P 〈 0. 05 ）. Conclusion Cy-3-g effectively promotes platelet apoptosis by affecting the proteins expression of BCL2 family.

关 键 词：花色苷 血小板凋亡 BCL2家族 

分 类 号：R151.41[医药卫生—营养与食品卫生学]