甲基乙二醛对胰腺癌PANC-1细胞增殖及凋亡蛋白表达的影响  

Effect of methylglyoxal on proliferation of human pancreatic carcinoma(PANC-1) cells and expression of apoptotic proteins

作　　者：张涓娟[1,2] 蒲宇 李勇[1,2] 沈成义[1,2] 张小明[1,2] 

机构地区：[1]川北医学院医学影像四川省重点实验室 [2]川北医学院附属医院放射科,四川南充637000

出　　处：《中华临床医师杂志（电子版）》2013年第18期126-128,共3页Chinese Journal of Clinicians(Electronic Edition)

基　　金：国家自然科学基金(81271643);中央财政支持地方高校专项资金(医学影像创新团队)

摘　　要：目的探讨甲基乙二醛(MGO)对胰腺癌PANC-1细胞增殖影响及其作用机制。方法培养PANC-1细胞,用四甲基偶氮唑蓝(MTT)比色法检测MGO对PANC-1细胞增殖的影响;Hoechst33258染色细胞后显微镜下观察MGO处理后PANC-1细胞形态变化,Western blot检测凋亡家族蛋白Bcl-2、Bax和Caspase-3的表达变化。结果 MTT检测发现MGO对人胰腺癌PANC-1有明显的抑制作用,且在一定程度上呈剂量时间依赖性;MGO处理48 h后的PANC-1细胞在Hoechst33258染色后荧光显微镜下呈现典型的凋亡细胞核固缩表现;Western blot检测表明MGO能显著降低Bcl-2蛋白表达量而提高Bax和Caspase-3蛋白表达量。结论 MGO能够有效抑制人胰腺癌PANC-1细胞增殖并诱导其凋亡,其作用机制可能是通过作用凋亡信号通路调控凋亡家族蛋白Bcl-2,Bax和Caspase-3表达进而诱导PANC-1细胞凋亡。Objective To study the influence of methylglyoxal on human pancreatic carcinoma PANC-1 cells proliferation and possible mechanism. Methods Cell proliferation was evaluated using MTT method;Hoechst33258 staining assay was used to observe MGO-induced morphological changes;Changes for Bcl-2 and Bax expression level were assessed using Western blot. Results Comparing with control group, PANC-1 cells proliferation was suppressed by methylglyoxal. The growth inhibition induced by MGO was in a dose-dependent manner（P〈0.01）. Apoptosis of the PANC-1 cells was observed after exposure to MGO, which was verified by morphological changes and increased proportion of Hoechst positive cells. The expression level of Bcl-2 was significantly decreased compared with the control group, but that of Bax was higher in the former than that in the latter, as well as the expression level of Caspase-3. Conclusion MGO could inhibit PANC-1 cells proliferation significantly and induce the apoptosis via regulating the expression level of Caspase-3 and Bcl-2 family proteins.

关 键 词：胰腺肿瘤 BCL-2相关X蛋白质 甲基乙二醛 CASPASE-3 BCL-2 

分 类 号：R735.9[医药卫生—肿瘤]