微小RNA-126和微小RNA-7在食管鳞状细胞癌中的表达及临床意义  

Expressions and clinical signfficances of microRNA-126 and microRNA-7 in esophageal squamons cell carcinoma

作　　者：王江峰[1] 凌志强[1] 毛伟敏[1] 

机构地区：[1]浙江省肿瘤研究所,杭州310022

出　　处：《国际肿瘤学杂志》2013年第12期936-940,共5页Journal of International Oncology

基　　金：卫生部科研基金（WKJ2010-2-004）;浙江省重大科技专项计划项目（2011C13039-1）

摘　　要：目的探讨微小RNA-126（miR-126）和微小RNA-7（miR-7）在食管鳞状细胞癌（ESCC）中的表达情况及其与ESCC临床病理特征、预后之间的关系。方法采用实时荧光定量聚合酶链反应法检测116例ESCC组织和癌旁正常组织中miR-126和miR-7的表达差异，并对miR-126和miR-7表达情况与ESCC患者临床病理特征、预后的相关性进行统计学分析。结果miR-126不同程度低表达病例数为73（62．9％），正常表达病例数为35（30．2％），高表达病例数为8（6．9％）；miR-7不同程度低表达病例数为52（44．8％），正常表达病例数为35（30．2％），高表达病例数为29（25．0％）。miR-126和miR-7表达降低组无瘤生存期均较非降低组短（r=4．268，P〈0．05；x2=4．993，P〈0．05）；miR-126低表达与ESCC肿瘤位置、家族史和饮酒相关（Xz=14．564，P〈0．05,x2=5．691，P〈0．05；X2=4．971，P〈0．05），与性别、年龄、分化程度、浸润程度、淋巴转移、吸烟不相关（均P〉0．05）；miR-7低表达与ESCC的临床病理特征均不相关（均P〉O．05）。结论miR-126和miR-7的低表达可能与ESCC患者预后相关，具有一定的临床检测意义。Objective To detect the expressions of microRNA-126 （miR-126） and microRNA-7 （miR-7） in esophageal squamous cell carcinoma （ESCC） and to analyze their correlations with clinicopatho- logic features and prognosis of ESCC. Methods The expressions of miR-126 and miR-7 in 116 ESCC samples and matched normal tissue samples were detected by real-time PCR. Statistical analysis was used to find the relationships among the expressions of miR-126 and miR-7, pathological characteristics and prognosis. Results Low expression, normal expression and high expression of miR-126 were found in 73 （62.9%）, 35 （30.2%） and 8 （6.9%） carcinoma samples respectively. Low expression, normal expression and high expression of miR-7 were found in 52 （44.8%）, 35 （30.2%） and 29 （25.0%） carcinoma samples respectively. The dis- ease-free survival in patients with low expression of miR-126 and miR-7 was shorter than that in patients with non-low expression （X2 = 4.268, P 〈 0.05 ; X2 = 4. 993, P 〈 0.05 ）. The low expression of miR-126 was corre- lated with tumor location, family history and drinking （X2 = 14. 564, P 〈 0.05 ; X2 = 5. 691, P 〈 0.05 ; X2 = 4. 971, P 〈 0.05）, but was uncorrelated with gender, age, differentiation, infiltration, lymphatic metastasis and smoking （ all P 〉 0.05）. The low expression of miR-7 was uncorrelated with pathological characteristics of ESCC （ all P 〉 0.05 ）. Conclusion The low expressions of miR-126 and miR-7 may be related to the progno- sis of patients with ESCC, and have a certain clinical detection significance.

关 键 词：食管肿瘤 预后 微小RNA-126 微小RNA-7 

分 类 号：R735.1[医药卫生—肿瘤]