基于石墨/聚吡咯电极的葡萄糖生物传感器  

A glucose biosensor based on a graphite / polypyrrole composite electrode

作　　者：闫长领[1] 刘璟[1] 郭玉娟[1] 王公轲[1] 卢雁[1] 

机构地区：[1]河南师范大学化学化工学院,新乡453007

出　　处：《分析试验室》2014年第1期17-20,共4页Chinese Journal of Analysis Laboratory

基　　金：国家自然科学基金项目(21173071;21071047)资助

摘　　要：采用电化学聚合技术,在掺杂的导电聚吡咯薄膜修饰过的铅笔芯电极上,吸附葡萄糖氧化酶制备葡萄糖生物传感器。首先在含有0.1 mol/L吡咯和0.01 mol/L HCl的溶液中,于0.7 V恒电位下吡咯单体氧化聚合,在铅笔芯电极表面形成聚吡咯薄膜;然后将葡萄糖氧化酶吸附在修饰过的电极上制备出葡萄糖氧化酶-聚吡咯-铅笔芯电极电流型生物传感器。实验考察了吡咯聚合时间、聚合温度、葡萄糖氧化酶吸附量、检测电压以及干扰物对传感器性能的影响。实验结果表明,在优化条件下,传感器的灵敏度为17.78μA/mmol/L,线性范围0.8 mmol/L,线性相关度R=0.9918,响应时间小于16 s,检测下限为18.75μmol/L,有较强的抗干扰能力。A glucose biosensor, based on a polypyrrole/graphite electrode was tabncated by eiectrocnemlcal technology. Polypyrrole film was deposited onto a pencil-core electrode by electrochemical polymerization in the solution containing 0. 1 mol/L pyrrole and 0. 01 moL/L HCl at 0. 7V. Glucose oxidase was adsorbed onto the polypyrrole film. The influences of polymerization time, polymerization temperature, glucose oxidase adsorption, work potential and interferences on the performance of the biosensor were investigated. The experimental results show that under the optimal conditions, the sensitivity of the sensor is 17.78 μA/mmol/L, with a linear range of 00. 8 mmol/L, a detection limit of 18.75 μmol/L, a correlation coefficient of 0. 9918, strong anti-interference a- bility and the response time of less than 16 s.

关 键 词：聚吡咯薄膜 电化学聚合 葡萄糖生物传感器 抗干扰 

分 类 号：O657.1[理学—分析化学]