同位素稀释-气相色谱-串联质谱法测定植物油中的15种欧盟优控多环芳烃  

Determination of polycyclic aromatic hydrocarbons(PAHs) in vegetable oil by gas chromatography coupled with triple quadrupole mass spectrometry based on an isotope dilution

作　　者：王建华[1] 杨君[2] 刘靖靖[1] 姜瑛[1] 

机构地区：[1]山东出入境检验检疫局技术中心,青岛266002 [2]青岛大学化学化工学院,青岛266071

出　　处：《分析试验室》2014年第1期48-53,共6页Chinese Journal of Analysis Laboratory

基　　金：国家质检总局科技计划项目(2011IK222)资助

摘　　要：建立了凝胶渗透色谱（GPC）净化、气相色谱．串联质谱（GC—MS／MS）同时测定植物油中15种欧盟优控多环芳烃（PAHs）残留的分析方法。植物油样品加入同位素内标混合溶液后经乙腈一丙酮（体积比为60：40）混合提取、GPC净化，GC—MS／MS采用选择反应监测模式（SRM）采集数据后进行定性／定量分析；优化了影响分析结果的仪器条件。采用内标法定量，植物油样品中添加回收率在70．3％～123．5％之间，相对标准偏差为1．5％-9．9％，定量限为0．276～0．436μg/kg，相关系数均大于O．9985。An efficient and selective analytical method for the determination and the quantification of 15 EU pri- ority polycyclic aromatic hydrocarbons （PAHs） in vegetable oil has been developed. The samples were extracted by acetonitrile/acetone （V： V=60：40） followed by a purification with gel permeation chromatography （GPC）. Identification and quantification were performed using GC-MS/MS with selected reaction monitoring （SRM） mode, with an isotope dilution approach. The novel combination of selective extraction followed by purification provides highly purified analytes combined to a fast and automated method. Recoveries were between 70. 3 % and 123.5%. Relative standard deviations were between 1.5% and 9. 9%. Limits of detection were between 0. 276 -0. 436 μg/kg.

关 键 词：植物油 凝胶渗透色谱 同位素稀释 气相色谱 三重四极杆串联质谱 多环芳烃 

分 类 号：O657.63[理学—分析化学]