超高效液相色谱-串联质谱法测定辣椒制品中酸性橙Ⅱ  

Determination of acid orangeⅡin pepper products by ultra performance liquid chromatography-tandem mass spectrometry

作　　者：宿书芳[1] 王骏[1] 霍艳敏[1] 于文江[1] 薛霞[1] 张卉[1] 

机构地区：[1]山东省产品质量监督检验研究院,国家加工食品质量监督检验中心(山东),山东省材料化学安全检测技术重点实验室,济南250103

出　　处：《分析试验室》2014年第1期116-120,共5页Chinese Journal of Analysis Laboratory

摘　　要：建立了辣椒制品中酸性橙Ⅱ的超高效液相色谱-串联质谱分析方法。样品用乙腈提取，WAX弱阴离子固相萃取柱净化浓缩，经超高效液相色谱分离，三重四极杆质谱电喷雾电离（ESI），负离子多反应监测模式检测。结果表明，在1—200ng／mL质量浓度范围内，线性相关系数大于0．998；加标回收率为83．2％～113．2％，相对标准偏差为0．7％-7．2％。检出限分别为1．0μg／kg（辣椒油及辣椒酱）和2．0μg／kg（辣椒粉）。方法适用于辣椒制品中酸性橙Ⅱ的测定。A method for the determination of acid orange II in hot pepper products by ultra performance liquid chromatography-tandem mass spectrometry was established. The samples were extracted with acetonitrile and pu- rifled by WAX SPEs. The separation was target compound was detected by MS/MS carried out by ultra performance liquid c system with electrospray ionization （ESI hromatography （UPLC）. The ） under multiple reaction mo-nitoring （MRM） mode. The calibration curve showed good linearity in the range of 1 - 200 ng/mL with correla- tion coefficient better than 0. 99. The average recoveries ranged from 83.2% to 113.2% at the spiking levels of 2 ~ 200 μg/kg with RSDs of 0. 7% ~ 7.2%. The limit of quantitation （LOQ） was 1.0 μg/kg for chili oil and chili sauce and 2. 0 μg/kg for chili power, respectively. The method is fast-speed, high sensitivity and could be applied to quantitative determination of acid orange II in pepper products.

关 键 词：酸性橙Ⅱ 辣椒制品 超高效液相色谱-串联质谱法 

分 类 号：O657.63[理学—分析化学]