三氧化二砷对人肝癌SMMC-7721细胞的体内外作用及可能的机制  

Effect of arsenic trioxide on SMMC-7721 cells of patients with hepatoma and its potential mechanism in vivo and vitro

作　　者：朱正[1] 沈宇[1] 李德春[2] 

机构地区：[1]南京医科大学附属江苏盛泽医院普外科,江苏苏州215228 [2]苏州大学附属第一医院普外科,江苏苏州215006

出　　处：《实用临床医药杂志》2013年第21期27-32,共6页Journal of Clinical Medicine in Practice

基　　金：中国高校医学期刊临床专项资金(11321146)

摘　　要：目的观察三氧化二砷(As2O3)在体内外对人肝癌SMMC-7721细胞抗肿瘤作用。方法不同浓度的As2O3作用于SMMC-7721细胞48 h,采用MTT法测定细胞的存活率;荧光显微镜观察细胞形态变化;FITC-AnnexinⅤ/PI双标记检测SMMC-7721细胞的凋亡;比色法检测Caspase-3活性变化。用As2O3在SMMC-7721肝癌细胞荷瘤裸鼠的瘤体内进行注射治疗,观察肿瘤生长变化,12 d后处死裸鼠,摘除瘤体,称瘤重,并通过免疫组化法检测Bcl-2、Bax和Caspase-3的表达。结果 As2O3能显著抑制SMMC-7721细胞的增殖,半数抑制率浓度为(18.17±2.10)μmol/L;显微镜下可见有典型的细胞凋亡形态学改变,As2O3处理组SMMC-7721细胞凋亡率与对照组相比显著增加,As2O3可提高SMMC-7721细胞的Caspase-3活性。As2O3瘤内注射肝癌细胞株SMMC-7721裸鼠移植瘤后,能显著抑制肿瘤生长,瘤重的抑制率可达52.37%,免疫组化结果显示As2O3能明显上调与细胞凋亡相关因子Bax和Caspase-3的表达和下调Bcl-2的表达。结论 As2O3能抑制肝癌SMMC-7721细胞的生长,诱导SMMC-7721细胞凋亡;As2O3能抑制SMMC-7721细胞的体内致瘤能力。Objective To observe the anti-tumor effect of arsenic trioxide （As203） on SMMC-7721 cells of patients with hepatoma in vivo and vitro. Methods Methyl Thiazolyl Tetrazoli- um （MTY） method, fluorescence microscope, FITC-AnnexinV/PI double-tagging method and colori- metry were used to detect the survival rate, morphological change, apoptosis and Caspase-3 activity change of SMMC-7721 cells in different concentration of A%O3 for 48 h, respectively. AszO3 was in- jected into the transplanted tumors in nude mice with SMMC-7721 hepatoma cells to observe the growth of tumors. The mice were sacrificed after 12 d, followed by the extirpation and weighing of the tumors. Then Bcl-2, Bax and Caspase-3 expression were detected by immunohistochemistry. Results As203 could obviously inhabit the proliferation of SMMC-7721 ceils, with inhibition concentration （IC） being （18.17 ± 2. 10） μmol/L. Microscope showed typical morphological change of cell apoptosis and As203 treatment group was evidently higher than control group in apoptosis rate of SMMC-772 cells, suggesting that As203 could improve SMMC-772 cell activity. Internal injection of As203 into trans- planted tumors in nude mice with SMMC-7721 hepatocellular lines could remarkably inhabit the growth of tumors with inhabiting rate being52.37%, and immunohistochemistry also revealed that As203 could apparently up-regulate cell apoptosis-related Bax and Caspase-3 expression, and down-regulate Bcl-2 expression. Conclusion As203 can inhabit the growth and in vivo oncogenesis of hepatoma SMMC-7721 cells, and induce SMMC-7721 cell apoptosis.

关 键 词：三氧化二砷 凋亡 SMMC-7721细胞 体内成瘤 

分 类 号：R735.7[医药卫生—肿瘤]