原癌基因蛋白质C-erb B-2及增殖细胞核抗原在结肠癌中的定量检测及临床意义  

Quantitative Study on C-erb B-2 and Proliferation of Cell Nuclear Antigen in Colorectal Cancer and its Clinical Significance

作　　者：晋雯[1] 林志武[2] 高美钦[1] 杨代兴[1] 

机构地区：[1]福建医科大学病理解剖学教研室,福州350004 [2]福建省立医院肿瘤科,福州350001

出　　处：《福建医科大学学报》2000年第4期341-343,共3页Journal of Fujian Medical University

基　　金：福建省教委科研基金资助!(JA970 83 )

摘　　要：目的　探讨原癌基因蛋白质 C- erb B- 2及增殖细胞核抗原 (PCNA )在结肠癌中含量及与结肠癌生物学行为的关系。　方法　利用免疫组织化学及自动化图像分析技术定量检测 C- erb B- 2及 PCNA在 10例正常肠粘膜 ,30例结肠腺瘤 ,5 3例结肠癌中的表达。　结果　阳性总面积、积分光密度、平均光密度、阳性率 1 、平均光密度 1及半定量测定的阳性率等 6个参数值均按正常肠粘膜→ 级不典型增生腺瘤→ 级不典型增生腺瘤→ 级不典型增生腺瘤→腺癌的顺序呈递变趋势 ,伴有淋巴结转移的腺癌组中 ,上述各参数值明显增高。　结论　 C- erb B- 2及 PCNA一起可作为判断肿瘤的恶性程度及评价预后的指标 ,应用图像分析技术对结肠癌及癌前病变进行多指标多参数的计量分析 ,具有鉴别诊断的重要意义 ,为结肠癌的早期诊断提供客观依据。Objective\ To evaluate C\|erb B\|2 expression and proliferation of cell nuclear antigen(\{PCNA\}) in colorectal tumor and their potential usage in early diagnosis of colorectal cancer.\ Methods\ 10 normal colonic mucosae,30 colorectal adenomas and 53 colorectal carcinomas with complete follow\|up data were examined for PCNA and C\|erb B\|2 with quantitative computed processed imaging analysis.\ \{Results\}\ The values of most of the parameters(density,average of density and positive rate of C\|erb B\|2;average of density and positive rate of PCNA) increased in accordance with the normal mucosa\|adenoma\|adenocarcinoma sequence,notably indicating a tendency reflecting the progression of colorectal malignancy.\ Expression of C\|erb B\|2 and PCNA in colorectal cancer was related to the neoplasm's differentiated degree and metastasis.\ Conclusion\ C\|erb B\|2 and PCNA may be useful in evaluating malignancy degree and prognosis of colorectal tumor. \;

关 键 词：结肠癌 原癌基因蛋白质 C-ERB B-2 PCNA 诊断 

分 类 号：R735.35[医药卫生—肿瘤]