正常垂体及垂体瘤组织中新的全长cDNA的克隆  

New full length cDNA cloned from normal pituitary and pituitary tumors

作　　者：宋怀东[1] 彭永德[1] 胡仁明[1] 黄秋花[2] 吴昕彦[2] 周隽[2] 任双喜 高国峰[1] 王立[2] 毛羽丰[1] 余亚萍 戴蒙[1] 徐淑华[2] 范惠泳 沈宇 付刚 陈名道[1] 张庆华[2] 陈家伦[1] 

机构地区：[1]上海第二医科大学附属瑞金医院上海市内分泌研究所,200025 [2]上海第二医科大学附属瑞金医院上海血液学研究所 [3]国家人类基因组南方研究中心

出　　处：《中华医学杂志》2000年第12期890-892,共3页National Medical Journal of China

基　　金：国家"八六三"高科技研究基金资助项目(102-10-01-02);国家自然科学基金资助项目(39870311);国家卫生部B类科技攻关资助项目(98-2-285);国家人类基因组南方研究中心资助项目;史克必成公司资助项目

摘　　要：目的　从正常垂体及垂体瘤组织中克隆新的全长cDNA。方法　垂体cDNA文库构建 ,大规模表达序列标签 (ESTs)产生及生物信息学处理 ,芯片拼接及cDNA末端快速扩增 (RACE)。结果　从正常垂体及垂体瘤组织分别克隆 41条和 2 6条新的全长cDNA ,共 6 7条。在这些基因中 ,与内分泌功能相关者 16条 ;至少有 2个是具有典型信号肽结构的分泌蛋白 ;3个是具有典型穿膜结构的膜蛋白。在 5个参与激素信号转导的新基因中 ,至少有 2个可能参与酪氨酸激酶型受体的信号转导过程。结论　通过ESTs手段 ,短期内克隆到 6 7条新的全长cDNA。在这些新基因中 ,有些可能在垂体的生理及病理过程中起重要作用。Objective To clone new full length cDNA from normal pituitary and pituitary tumors. Methods cDNA libraries of human normal pituitary and pituitary tumors were constructed, and large-scale sequencing and bioinformatics were used. Silico cloning and rapid amplification of cDNA ends (RACE) were also used. Results 67 new full length cDNAs were cloned: 41 were cloned from normal pituitary, and 26 from pituitary tumors. Of the 67 cDNAs, 16 were related with endocrine function; at least 2 with a classical signal peptide were secretory protein, and 3 with classical transmembrane domains were transmembrane protein. Among the 5 cloned new full length cDNAs involved in signal transduction pathway of hormone, at least 2 participated in signal transduction pathway of receptors with tyrosine kinase activition. Conclusion During short time, 67 new full length cDNA were cloned from normal pituitary and pituitary tumors by large-scale producing expressed sequence tags. Some of them may play an important role in the regulation of physiological and pathological processes of pituitary.

关 键 词：垂体 垂体瘤 CDNA 

分 类 号：R736[医药卫生—肿瘤]