外源一氧化氮和过氧化氢调节菊苣盐适应性  

Exogenous nitric oxide and hydrogen peroxide regulate the acclimation of chicory (Cichori-um intybus) to salt stress

作　　者：谷文英[1] 莫平华[2] 杨江山 赵国琦[1] 高洪文[4] 

机构地区：[1]扬州大学动物科学与技术学院,江苏扬州225009 [2]扬州大学兽医学院,江苏扬州225009 [3]百绿天津国际草业有限公司,北京100025 [4]中国农业科学院北京畜牧兽医研究所,北京100193

出　　处：《生态学杂志》2014年第1期89-97,共9页Chinese Journal of Ecology

基　　金：现代农业产业技术体系建设专项;江苏高校优势学科建设工程项目;江苏省草学重点学科建设项目资助

摘　　要：研究外源性一氧化氮（NO）供体硝普钠（SNP, 0.1 mmol·L-1）和过氧化氢（H2O2, 0.5 mmol·L-1）对NaCl（210 mmol·L-1）胁迫下菊苣（Cichorium intybus）幼苗生长、抗氧化酶活性和逆境蛋白的影响。结果表明：与空白对照相比，盐胁迫导致菊苣幼苗的根长和鲜重显著降低；丙二醛（MDA）含量显著升高（P〈0.05）；超氧化物歧化酶（SOD）和过氧化氢酶（CAT）活性减弱，而过氧化物酶（POD）活性增强；热激蛋白90（HSP 90）和脱水蛋白（CiDHN1）mRNA的相对表达量增加，CiDHN1含量在2~48 h内持续升高。与盐胁迫对照相比，SNP预处理缓解了盐胁迫对菊苣幼苗生长的抑制；使幼苗MDA含量显著下降；SOD、POD和CAT活性显著增强（P〈0.05），SOD和POD同工酶谱带增多；并使HSP90和CiDHN1 mRNA的相对表达量和蛋白含量均进一步增加；H2O2预处理也具有类似的效应。这说明SNP和H2O2预处理对菊苣幼苗盐胁迫的缓解效应与其上调抗氧化酶的活性和逆境蛋白的表达有关。Chicory （Cichorium intybus） seedlings were employed to study the impacts of exogenous nitric oxide （NO） donor （sodium nitroprusside （SNP）, 0.1 mmol·L-1） or hydrogen peroxide （H2O2, 0.5 mmol·L-1） on their growth, antioxidant enzyme activities and stress protein expression under salt stress （210 mmol·L-1 of NaCl）. The results showed that root length and fresh weight of chicory seedlings were obviously decreased under salt stress compared with the control, whereas the content of malondialdehyde （MDA） sig nificantly increased （P〈0.05）. In addition, isoenzyme analysis showed that the activities of superoxide dismutase （SOD） and catalase （CAT） were weakened while peroxidase （POD） activity obviously increased under salt stress. The data from qPCR analysis suggested that the relative quantity of HSP 90 mRNA and CiDHN1 mRNA increased under salt stress, but the data from ELISA indicated that only the content of CiDHN1 gradually increased in a timedependent manner （2-48 h）. Compared with the single salt stress, the inhibition on root length and fresh weight of plants induced by salt stress was apparently alleviated by SNP pretreatment, with a dramatic decline of MDA content, an obvious rise in activities of SOD, POD and CAT and an increase in numbers of SOD and POD isoenzymes. Moreover, qPCR analysis indicated that SNP pretreatment further increased the relative quantity of HSP 90 mRNA and CiDHN1 mRNA, and the contents of HSP 90 and CiDHN1 in chicory seedlings. The effects of H2O2 pretreatment were similar to those of SNP pretreatment. These data suggested that both of SNP and H2O2 were involved in alleviating adverse effects of salt stress on chicory seedlings by increasing antioxidant enzyme activities and stress protein expression.

关 键 词：菊苣 一氧化氮 硝普钠 过氧化氢 抗氧化酶 逆境蛋白 

分 类 号：S636.9[农业科学—蔬菜学]