荧光原位杂交技术在肺癌痰细胞学诊断中的应用  

Application of fluorescence in situ hybridization (FISH) in sputum cytologic diagnosis of lung cancer

作　　者：贾东辉[1] 张智慧[1] 刘树范[1] 程书钧[1] 

机构地区：[1]中国医学科学院中国协和医科大学肿瘤研究所肿瘤医院临床细胞学室,北京100021

出　　处：《中华肿瘤杂志》2000年第6期477-479,共3页Chinese Journal of Oncology

基　　金：国家重点基础研究经费资助项目(G1998051200)

摘　　要：目的　探讨荧光原位杂交技术 (FISH)在肺癌痰细胞学诊断中的应用。方法　用 7号、11号、17号、X染色体特异的着丝粒DNA探针 ,对 30例肺癌痰标本进行双色FISH。结果　 30例痰标本中 ,有 2 3例为阳性痰 ,7号、17号、X、11号染色体出现超二倍体改变者分别为 6 5 .2 % (15 / 2 3)、6 0 .9% (14/ 2 3)、5 2 .2 % (12 / 2 3)和 39.1% (9/ 2 3)。在 7例细胞学诊断为可疑癌的痰标本中 ,出现 7号染色体超二倍体改变者达到 5 7.1% (4 / 7)。结论　FISH不仅可以检测痰标本中的异倍体肿瘤细胞 。Objective To study the numerical chromosomal abnormalities of cells in sputum from patients with lung cancer by dual fluorescence in situ hybridization (FISH). Methods Thirty sputum samples from lung cancer patients were examined by FISH with centromere DNA probes of chromosome 7, 11, 17 and X. Results In 23 positive sputum samples studied, the frequency of hyperdiploid of chromosome 7, 17, X and 11 was 65.2% (15/23), 60.9% (14/23), 52.2% (12/23) and 39.1% (9/23), respectively. Hyperdiploidy of chromosome 7 was found in 4 of 7 sputum samples which were cytologicaly suspicious of cancer cells.Conclusion FISH can detect aneuploid malignant cells in sputum and be used as a complementary technique to cytologic diagnosis of lung cancer.

关 键 词：肺肿瘤 原位杂交 痰标本 超二倍体 

分 类 号：R734.2[医药卫生—肿瘤]