胡黄连苷Ⅱ预处理对大鼠离体心脏缺血再灌注的影响  

Effects of Picroside Ⅱ Preconditioning on Myocardial Ischemia Reperfrusion Injure in Isolated Rats Heart

作　　者：吴楠[1] 李雯娜[1] 吕岩[1] 舒雯琪 贾大林[1] 

机构地区：[1]中国医科大学附属第一医院心内科,沈阳110000

出　　处：《医学研究杂志》2013年第12期46-49,共4页Journal of Medical Research

基　　金：辽宁省科技计划项目(2009225051-1)

摘　　要：目的探讨胡黄连苷Ⅱ预处理对大鼠离体心脏缺血再灌注的影响。方法健康Wistar大鼠32只,随机分为对照组(control组)、1μmol/L胡黄连苷Ⅱ预处理组(1-P)、10μmol/L胡黄连苷Ⅱ预处理组和大剂量胡黄连苷Ⅱ预处理组(HP)。采用Langendorff离体心脏流灌装置,行缺血30min,再灌注120min,制备心肌缺血再灌注模型。胡黄连苷Ⅱ预处理于缺血前15min分别以含有1μmol/L和10μmol/L的胡黄连苷ⅡKH液灌注。记录各组心功能指标,测定心肌梗死面积,测定冠脉灌流出液中超氧化物歧化酶(SOD)、丙二醛(MDA)含量。结果 LP组和HP组明显加快心率(HR)、提高左心室发展压(LVDP)和左心室内压最大上升及下降速率(±dp/dt max),缩小心肌梗死范围,提高SOD的活性,降低MDA含量,与对照组比较,差异有统计学意义(P<0.05),但HP组较LP组无统计学差异(P>0.05)。结论胡黄连苷Ⅱ预处理能减轻心肌缺血再灌注损伤,其心肌保护作用机制可能与胡黄连苷Ⅱ提高机体抗氧化应激损伤的能力有关。Objective To investigate the effects of picroside II preconditioning on myocardial ischemia reperfrusion injure in isola- ted rats heart. Methods Twentyfour rats were randomly separated into control group, low - dose picroside Ⅱ preconditioning group（LP） and high - dose picroside Ⅱ preconditioning group（ HP）. Isolated hearts were linked to Langendorff device with 30 minutes isehemia and 120 minutes reperfusion to prepare myocardial ischemia reperfusion models. Picroside ]] preconditioning was induced by perfusion with Krebs - Henseleit containing picroside ]I at I/xmol/L and 10p^mol/L respectively before 15 minutes ischemia. The changes of cardiac function of all groups, myocardial infarct size, SOD activity and MDA content in coronary perfusion fluid were determined. Results LP group and HP group significantly accelerated HR, improved LVDP and dp/dt reduced myocardial infarct size, enhanced SOD activi- ty and decreased MDA content compared with control group （ P 〈 0.05） , but no significant difference was seen between LP group and HP group（P 〉 0.05）. Conclusion Picroside ]] preconditioning attenuates myocardial ischemia reperfrusion injure, which is involved in en- hancing the body＇s resistance to the oxidative stress injury.

关 键 词：胡黄连苷Ⅱ 缺血预处理心肌缺血再灌注损伤氧化应激 

分 类 号：R-332[医药卫生]