三黄抗氧化方抑制乳腺癌MCF-7细胞氧化应激与增殖的实验研究  

Experimental Rresearch of Effects of Anti-oxidative Stress of Breast Cancer MCF-7 Cells and the Mechanism with Traditional Chinese Decoction of Sanhuangkang Oxide

作　　者：张晓清[1] 卞卫和[1] 李琳[1] 姚昶[1] 任晓梅[1] 应语[1] 郭檬檬[2] 曹东阳[2] 陈灵[2] 

机构地区：[1]江苏省中医院乳腺科,南京210000 [2]南京中医药大学

出　　处：《医学研究杂志》2013年第12期64-67,共4页Journal of Medical Research

基　　金：江苏省中医药领军人才基金资助项目(LJ2009002)

摘　　要：目的探查三黄抗氧化方抑制乳腺癌MCF-7细胞氧化应激与增殖的疗效。方法将实验分为空白组：MCF-7细胞培养；实验组按黄芪、制大黄、片姜黄比例分：实验组1：1：1：1，实验组2：3：1：1，实验组3：6：1：l；氧化应激组：以过氧化氢模拟细胞氧化应激状态；乙酰谷氨酸液（NAC）还原组：氧化应激组中加入NAC还原应激反应；免疫荧光法评价其抗MCF-7细胞产生反应性氧化物疗效及ELISA法评价氧化应激后细胞培养液中VEGF含量；同时MTT法评价三黄抗氧化方抑制MCF-7细胞的增殖率。结果实验组2抗氧化氧化应激结果优于其他两组，并显著降低细胞培养液中VEGF水平；同时各实验组MCF-7细胞增殖率得到显著抑制并呈现明显的浓度依耐性，相对空白组，实验组1～3在8mg／ml浓度下分别抑制MCF-7细胞增殖达57％、67％及56％，实验组2的MCF-7细胞增殖抑制率显著高于实验组1与3，有统计学差异（P〈0．01）。结论三黄抗氧化方抑制MCF-7细胞氧化应激同时抑制其增殖率，并降低氧化应激引起的VEGF表达。Objective To explore the effects of anti - oxidative stress and suppressing proliferation rate of breast cancer MCF - 7 cells with traditional Chinese decoction of Sanhuangkang Oxide. Methods Blank group with only MCF -7 cells culture,3 experimental groups of experimental groupl with the ratios of Hangqi： Zhidahuang： Jianghuang to 1： 1： 1, followed by 3： 1：1 and 6： 1：1 for experimen- tal group2 and 3, oxidative group with hydrogen peroxide stimulation and deoxidation group with NAC interfered were established for the research. The efficiency of suppressing reactive oxide produced from MCF - 7 cells was measured by immunofluoreseenee technique. The VEGF level was measured in the super fluid of cell culture with ELISA experiment. The cell proliferative rate of Sanhuangkang oxide was measured by MMT. Results The group 2 was superior to other two groups in the aspect of anti - oxidative stress and reducing the VEGF level in the super fluid of cell culture. Compared with control group, the groups could significantly suppress the proliferation rate of MCF -7 cells, which depended on concentrations. The proliferation rate of groups were 57% , 67% and 56% , respectively in the concentra- tion of 8mg/ml. Significant differences gotten from the experimental group 2 with the highly inhibition of MCF - 7 cells proliferation of p value less than 0.01. Conclusion Traditional Chinese decoction of Sanhuangkang Oxide have the ability to inhibition of MCF - 7 cells proliferation, reducing the oxidative stress, followed by down regulating the secretion of VEGF.

关 键 词：三黄抗氧化方 乳腺癌 MCF-7细胞 氧化应激 

分 类 号：R737.9[医药卫生—肿瘤]