肺形侧耳高温后恢复期间海藻糖代谢途径研究  

Study on the Metabolic Pathway of Trehalose in Pleurotus pulmonarius During Heat Stress Recovery

作　　者：刘秀明[1] 黄晨阳[1] 陈强[1] 邬向丽[1] 张金霞[1] 

机构地区：[1]中国农业科学院农业资源与农业区划研究所/农业部农业微生物资源收集与保藏重点实验室,北京100081

出　　处：《中国农业科学》2013年第24期5188-5195,共8页Scientia Agricultura Sinica

基　　金：现代农业产业技术体系建设专项资金(CARS-24)

摘　　要：【目的】探索食用菌对高温胁迫后恢复响应差异的机制,为食用菌的高温育种研究提供理论基础。【方法】以肺形侧耳热敏感型菌株CCMSSC 00494和耐热型菌株CCMSSC 00499为材料,通过测定高温胁迫后恢复培养期间菌丝体内海藻糖代谢相关酶活性和基因相对表达量的变化,研究海藻糖代谢途径的应激响应。【结果】高温胁迫后恢复培养期间,两株肺形侧耳菌株海藻糖含量迅速降至对照水平,CCMSSC 00494中海藻糖含量降低速率高于CCMSSC 00499。CCMSSC 00494中海藻糖-6-磷酸合成酶(TPS)活性随着处理时间的延长增加,而CCMSSC 00499中的TPS活性缓慢下降。两菌株中合成海藻糖反应方向海藻糖磷酸化酶(TP)活性急剧下降。降解海藻糖反应方向TP活性和中性海藻糖酶(NTH)活性分别在恢复前期和后期被激活,参与海藻糖水解。tps和tp基因的表达量变化与各自酶活性变化一致,但nth基因的高效表达与其在恢复初期的NTH活性下降不一致。【结论】高温胁迫后恢复培养期间,热敏感型菌株中TPS活性和tps基因表达量显著高于耐热型菌株,是两者海藻糖代谢途径中的最主要差异。[Objective] The objective of this study is to explore the recovery mechanism after heat response stress of mushroom, in order to provide a theoretical basis for breeding. [ Method ] The responses of trehalose metabolism at the level of enzyme activity and gene relative expression quantity during heat stress recovery period in two strains of Pleurotus pulmonarius, including the heat sensitive strain CCMSSC 00494 and heat tolerant strain CCMSSC 00499 were investigated. [Result] The results showed that the trehalose content of two strains rapidly decreased to the control level under heat stress recovery conditions, and the rate of decrease in heat sensitive strain CCMSSC 00494 was faster than that in CCMSSC 00499. During post-heat stress recovery period, trehalose-6-phosphate synthase （TPS） activity of CCMSSC 00494 increased with prolongation of treatment time, while it decreased slowly in CCMSSC 00499. And in both strains, trehalose phosphorylase （TP） activity in the direction of trehalose synthesis decreased suddenly, and TP activity in the direction of trehalose phosphorolysis and neutral trehalase （NTH） activity for trehalose hydrolysis were activated in the early and late period of recovery, respectively. In contrast with the case of tps and tp Genes, the increase of nth mRNA expression was not consistent with the elevation of neutral trehalase activity during heat stress in both strains. [Conclusion]The main difference between heat sensitive and heat tolerant strains is that the activities and mRNA expression levels of TPS in heat sensitive strain was significantly higher during heat stress recovery period.

关 键 词：肺形侧耳 海藻糖 高温后恢复培养 耐热性 

分 类 号：S646[农业科学—蔬菜学]