米根霉Fp-6利用红薯发酵生产L-乳酸  

The study on fermentation of L-lactic acid with sweet potato by Rhizopus oryzea Fp-6

作　　者：黄筱萍[1] 黄国昌[1] 印培民[1] 

机构地区：[1]江西省科学院微生物研究所,江西南昌330029

出　　处：《食品与发酵工业》2013年第12期114-118,共5页Food and Fermentation Industries

摘　　要：以高产淀粉的优质红薯品种为原料,采用生产菌株米根霉Fp-6研究不同的红薯预处理方式对高光学纯度L-乳酸发酵的影响,并进行了50 L发酵罐放大试验。结果表明:用制备的红薯淀粉为原料,产酸和发酵周期均与以玉米淀粉为原料相同。当红薯液化液中的有机N含量高于0.25 g/L时,产乳酸量和糖转化率明显下降,副产物富马酸和甘油浓度明显增加,菌体量显著增加。无机N对发酵产酸有促进作用。用去除红薯中部分有机N的液化液进行发酵,在50 L罐中其产乳酸量达91.3 g/L,糖转化率为81.28%,发酵周期48 h。Taking quality sweet potato varieties with high-yield starch as experimental material, the effects of dif- ferent pretreatment of sweet potato on fermentations of L-lactic acid with high optical purity by Rhizopus oryzea Fp-6 were studied, and the enlarge tests were carried out in 50 L fermentor. While comparing sweet potato starch to corn starch as experimental material, the results showed that there were no differences on the yield of L-lactic acid and fer- mentation period. When the concentration of organic N was higher than 0.25 g/L in the liquefied sweet potato solu- tion, the lactic acid yield and the conversion rate of sugar were decreased significantly, while the biomass and the content of by-products （fumaric acid and glycerol） were increased obviously. However, inorganic N had the role to facilitate lactic acid fermentation. In the fermentation test with the liquefied sweet potato solution which removed a part of organic N, the results showed that the yield of L-lactic acid was 91.3 g/L, and the conversion rate of sugar was as high as 81.28% , and the fermentation period was 48 h in 50 L fermentor.

关 键 词：米根霉Fp-6 红薯 乳酸发酵 

分 类 号：TQ921.3[轻工技术与工程—发酵工程]