一种优化的大鼠视网膜组织石蜡切片制作方法  

An Improved Method for Preparation of Paraffin Slices of Rat Retina

作　　者：杨明[1] 唐永赢 周媛[2] 李艳[2] 姜晓璇 曹霞[3] 马林昆[2] 

机构地区：[1]昆明医科大学第一附属医院,云南昆明650031 [2]昆明医科大学第二附属医院 [3]昆明医科大学第二附属医院中心实验室,云南昆明650011

出　　处：《昆明医科大学学报》2013年第12期5-8,共4页Journal of Kunming Medical University

基　　金：国家自然科学基金资助项目(31160206);云南省科技厅-昆明医科大学联合专项基金资助项目(2010CD199)

摘　　要：目的针对大鼠视网膜石蜡切片过程中易出现标本脱离及断裂分离的问题,寻找并优化大鼠视网膜石蜡切片的制作方法并评估效果.方法分别用10%多聚甲醛固定液;4%多聚甲醛固定液;单独固定及使用4%多聚甲醛、95%酒精、冰醋酸混合固定液(FAA固定液);预处理后再使用10%多聚甲醛分别固定等4种方法对大鼠视网膜组织进行固定,HE染色后显微镜下进行观察.结果单独使用10%多聚甲醛以及4%多聚甲醛固定均出现视网膜脱离及各层结构断裂分离现象,但经FAA固定液预处理的视网膜切片HE染色后颜色鲜明均匀,虽部分区域视网膜偶有脱离,但不易脱片,各层结构完整,未出现断裂分离.结论经FAA固定液及多聚甲醛处理过的视网膜石蜡切片固定效果明显优于单独使用多聚甲醛,甲醛浓度对HE染色结果无明显影响.Objective To solve the problem that the rat retina paraffin sections are easily exfoliated from the slides and each layer are easily separation and fracture, we need to find a way to improve the retina paraffin section method and evaluate the tissue fixation. Methods We used 4 fixation liquids including 10% paraformaldehyde, 4% paraformaldehyde, 4% paraformaldehyde and 95% alcohol and glacial acetic acid mixed liquid （FAA fixatiue solution ） combined with paraformaldehyde to fix the retinal tissue, and observed the fixation efficacy under microscope after HE staining. Results The effects of 10% paraformaldehyde and 4% paraformaldehyde fixed samples showed moderate separation and fracture of retina, but the HE staining retinal slices pre-treated by the FAA fixative solution had bright and uniform color, although occasionally some parts of the retina were exfoliated from the slide, but it was not easy to take off, and had complete structure without separation and rupture. Conclusion The retina paraffin section fixed by FAA ixafive solution with 4% paraformaldehyde is superior to pure paraformaldehyde, and the paraformaldehyde concentration has no obviously influence on HE staining resuhs.

关 键 词：视网膜 石蜡切片 固定方法 

分 类 号：R361.2[医药卫生—病理学]