利用BC_2群体定位大豆蛋白质含量QTL  

Detection of soybean protein content QTL with BC_2 backcross population

作　　者：陈明阳[1] 张金巍[1] 韩粉霞[1] 孙君明[1] 邹筱[1] 闫淑荣[1] 杨华[1] 张晶莹[1] 田玲[1] 南金平 

机构地区：[1]中国农业科学院作物科学研究所,国家农作物基因资源与基因改良重大科学工程,农业部北京大豆生物学重点实验室,北京100081

出　　处：《中国油料作物学报》2013年第6期617-623,共7页Chinese Journal of Oil Crop Sciences

基　　金：转基因生物新品种培育重大专项(2011ZX08004-004;2011ZX08004-003);国家科技支撑计划(2011BAD35B06)

摘　　要：进行大豆蛋白质含量相关的QTL定位可为分子标记辅助选择（MAS）育种和基因挖掘提供依据。本研究选用综合性状优良、蛋白质含量高的中黄13作轮回亲本，低蛋白质含量的东山69作供体亲本，构建了BC2F2、BC2F3回交导入系群体，采用SSR分子标记技术，获得含86个SSR标记、覆盖1 400.1cM、由19个连锁群组成的遗传连锁图谱a，利用完备区间作图法（ICIM），对BC2F2、BC2F3分离群体的蛋白质含量进行QTL分析。结果表明：在BC2F2家系中检测到3个蛋白质含量相关QTL，分布于A1、C1和J连锁群，表型贡献率分别为10.00%、7.07% 和9.23%；在BC2F3家系中检测到4个蛋白含量相关QTL，分布于B1、C1、I和J连锁群，表型贡献率分别为17.57%、3.46%、8.53%和11.80%。标记区间Satt396～Satt180和Sct_001～Satt654在BC2F2、BC2F3家系中被同时检测到，且Sct_001～Satt654内发现的QTL很可能是一个与蛋白质含量相关的新位点。Seed protein content, controlled by quantitative genes, is a very important trait for soybean. To identify the soybean quantitative trait loci （QTL） of seed protein content, which provides a base for MAS breeding and elite genes-discovering, a cross was made between the elite cultivar Zhonghuang 13, which exhibits a comprehensive excellent performance and has the higher protein content and the variety Dongshan 69 which has the lower protein content. The BC2F2 and BC2F3 backcross populations were further developed to identify protein composite interval mapping （ICIM）, two QTLs flanked by the marker interval Satt396~Satt180 and Sct_001~ Satt654 were both detected in the BC2F2 and BC2F3 families. In the BC2F2 families, 3 QTLs were found on A1, C1 and J linkage groups, which accounted for 10.00%, 7.07% and 9.23% phenotypic variance, respectively. In the BC2F3 families, 4 QTLs were identified on B1, C1, I and J linkage groups, which explained 17.57%, 3.46%, 8.53% and 11.80% phenotypic variance, respectively. Sct_001~Satt654 may be a new interval associated with protein content QTL.

关 键 词：大豆 蛋白质含量 QTL定位 回交群体 完备区间作图法 

分 类 号：S565.103[农业科学—作物学]