hrpZpsta和RIPs双价抗病基因转化大豆的研究  

Studies on transforming hrpZpsta and RIPs genes into soybean

作　　者：张琳[1] 李林[1] 李丹[1] 代力强[1] 马建[1] 曲静[1] 付永平[1] 王丕武[1] 

机构地区：[1]吉林农业大学,吉林长春130118

出　　处：《中国油料作物学报》2013年第6期624-629,共6页Chinese Journal of Oil Crop Sciences

基　　金：国家转基因生物新品种培育科技重大专项(2011ZX08004-004)

摘　　要：构建了含有hrpZpsta和RIPs的双价抗病基因植物表达载体,采用农杆菌介导法,以大豆子叶节为外植体,将双价抗病基因hrpZpsta和RIPs转入大豆品种吉农28中,以耐盐基因BADH作为筛选标记基因、筛选得到T1阳性植株18株;T1转基因植株PCR、Southern杂交及RT-PCR检测表明外源基因已成功整合到了大豆基因组中并可遗传给后代。This experiment was to construct the expression vectors which contain hrpZpsta and RIPs genes and transform these resistant genes into soybean genome via Agrobacterlum tumefaciens - mediated transformation meth- od. Soybean cuhivar Jinong 28 cotyledonary node was used as explant. The putative transgenic plants were screened with salt -tolerance gene BADH and 18 T1 positive transgenic progenies were obtained. The PCR, South- ern blot, RT - PCR analysis showed that, hrpZpsta and RIPs genes had been transformed into soybean genome and could be inherited in the next progeny.

关 键 词：harpZpsta基因 RIPs基因 大豆子叶节 农杆菌介导法 抗病 

分 类 号：Q786[生物学—分子生物学] S565.103[农业科学—作物学]