益气化瘀解毒方药对人肝癌HepG2细胞增殖和裸鼠移植瘤生长的影响  

Prescription form of Strengthening Qi and Clearing Stasis and Poison effect on HepG2 liver cancer cell proliferation and the influence of the nude mouse transplantation tumor growth

作　　者：曾普华[1] 郜文辉[2] 潘敏求[1] 朱克俭[1] 蒋益兰[1] 李勇敏[1] 唐珍[1] 

机构地区：[1]国家中医药管理局重点学科,湖南省中医药研究院附属医院肿瘤科,湖南长沙410006 [2]湖南中医药大学中医学院,湖南长沙410208

出　　处：《现代肿瘤医学》2014年第1期8-11,共4页Journal of Modern Oncology

基　　金：国家自然科学基金资助项目(编号:81001522);湖南省自然科学基金课题(编号:13JJ4138);首届中国中西医结合学会-金港榄香烯中医药肿瘤研究基金资助项目(编号:2010-F10)

摘　　要：目的:探讨益气化瘀解毒方药对人肝癌HepG2细胞增殖和裸鼠移植瘤生长的影响。方法:以益气化瘀解毒方含药血清干预HepG2细胞后,观察24、48、72小时后细胞增殖状况;建立人肝癌移植瘤模型,分为7组,分别以益气化瘀解毒方、黄芪、莪术、重楼、壁虎、顺铂等药物干预3周,并设空白对照组,观察移植瘤生长状况。结果:各剂量含药血清组与不含药血清组之间的吸光度值差异明显(P<0.05);高、低剂量组间比较有明显差异(P<0.05)。裸鼠体重比较:顺铂组用药前后差值变化与其它各组比较差异有统计学意义(P<0.05),其它组间差异比较不明显(P>0.05)。成瘤体积比较:给药后瘤体积及差值比较,各组与空白组均有明显差异(P<0.05);全方组分别与黄芪组、重楼组、壁虎组、空白对照组等比较有明显差异(P<0.05)。结论:益气化瘀解毒方含药血清能抑制人肝癌HepG2细胞的生长,呈现量效关系。整方组对荷瘤裸鼠体重无影响,益气化瘀解毒方全方较单味中药具有较强的抑瘤作用,其组方中药如莪术、重楼、壁虎均有一定程度抑瘤作用,其中莪术抑瘤作用较强;组方内主要单味中药发挥了协同抑瘤效应,使整方呈现出最强的抑瘤效应。Objective：To investigate the effects of Prescription form of Strengthening Qi and Clearing Stasis and Poison（SQCSP）of human hepatocellular carcinoma HepG2 cells proliferation and the influence of the nude mouse transplantation tumor growth.Methods：With SQCSP drug-containing serum intervention on HepG2 cells,observing cell proliferation condition after 24,48,72 hours.Human liver transplantation tumor model,were divided into 7 groups,respectively by drug intervention such as SQCSP,astragalus root,rhizoma zedoariae,paris polyphylla,gecko,cisplatin,to observe the transplanted tumor growth for 3 weeks.Results：The dose of medicated serum group and do not contain medicine absorbance differences between serum group significantly （P〈0.05）.Comparison between the high and low dose groups had significant difference （P〈0.05）.Nude mice weight comparison：cisplatin group were different before and after the change compared with other groups （P〈0.05）,no difference between the other groups （P〉0.05）.Drug delivery to tumors before and after product comparison：tumors and different comparison,after the treatment group and the blank group were significant different （P〈0.05）.All parties respectively with astragalus membranaceus group,paris polyphylla,gecko group and blank control group were significant different （P〈0.05）.The whole party and cisplatin group was no significant different （P〉0.05）.Conclusion：SQCSP medicated serum can inhibit the growth of human hepatocellular carcinoma HepG2 cells.The whole party group had no effect on body weight in a tumor-burdened nude mice,the whole party group has stronger role in tumor suppression compare with a single taste traditional Chinese medicine （TCM）,the formula of TCM such as rhizoma zedoariae,paris polyphylla,gecko has a certain degree tumor suppression function,including rhizoma zedoariae tumor suppression effect is stronger.Formula in the main single Chinese medicine played a coordination tumor suppression effect,the whole party

关 键 词：益气化瘀解毒方 HEPG2细胞 增殖 移植瘤 

分 类 号：R73-361[医药卫生—肿瘤] R735.7[医药卫生—临床医学]