热休克蛋白HSP27抑制食管鳞癌细胞的侵袭转移能力  

Heat shock protein HSP27 inhibits the invasion and metastasis of esophageal carcinoma cell lines

作　　者：薛琳[1] 杨蕾[2] 刘冰[1] 李红[1] 康建琴[1] 王彪落 梁树辉[1] 丁杰[1] 

机构地区：[1]第四军医大学西京消化病医院,肿瘤生物学国家重点实验室,陕西西安710032 [2]宝鸡市第二人民医院,陕西宝鸡721000

出　　处：《现代肿瘤医学》2014年第1期11-15,共5页Journal of Modern Oncology

基　　金：国家自然科学基金资助项目(编号:30900674;81272516;30973428;81090273;81090274);陕西省科技计划项目(编号:2011K12-03-06)

摘　　要：目的:探讨HSP27分子的表达水平与食管鳞癌高、低转移潜能细胞系侵袭转移能力的关系。方法:选用EC9706-H、EC9706-L和EC109-H、EC109-L两对食管鳞癌高、低转移潜能细胞系,利用细胞划痕实验检测不同细胞侵袭转移能力的差异;利用Western blot检测HSP27在不同转移潜能细胞中的表达水平;利用慢病毒转染技术上调HSP27低表达细胞中HSP27的表达,采用细胞划痕实验检测其侵袭转移能力的变化。结果:细胞划痕实验证实,EC9706-H细胞和EC109-H细胞的体外侵袭转移能力高于EC9706-L细胞和EC109-L细胞;Western blot实验结果显示,HSP27在EC9706-H细胞和EC109-H细胞中呈低表达,在EC9706-L细胞和EC109-L细胞中呈高表达;通过慢病毒转染技术上调EC9706-H细胞和EC109-H细胞中HSP27的表达,二者的侵袭转移能力明显降低。结论:HSP27与食管鳞癌的侵袭转移能力呈负相关,即HSP27高表达的食管鳞癌细胞其侵袭转移能力受到抑制。Objective：To explore the relationship between the expression of heat shock protein 27 （HSP27） and the invasive and metastatic potential of human esophageal squamous cell carcinoma （ESCC） cell lines.Methods：Scratch assays were performed to identify the differences and changes of invasive and metastatic ability in ESCC cell lines.Western blot were used to examine the differences of HSP27 expression in ESCC cell lines.Lentivirus infection technique was employed to up-regulate the expression of HSP27 in highly metastatic cells.Results：The invasive and metastatic potentials of EC9706-H and EC109-H cells were higher than the matched EC9706-L and EC109-L cells.The expression levels of HSP27 in EC9706-H and EC109-H cells were lower than the matched EC9706-L and EC109-L cells.The invasive and metastatic potentials of EC9706-H and EC109-H cells were significantly decreased after overexpression of HSP27.Conclusion：The expression level of HSP27 may be inversely correlated to the invasive and metastatic behavior of ESCC cell lines and up-regulating the expression of HSP27 can inhibit the invasion and metastasis ability of ESCC.

关 键 词：食管鳞癌 热休克蛋白27 侵袭 转移 

分 类 号：R73-362[医药卫生—肿瘤] R735.1[医药卫生—临床医学]