吡格列酮对肾小球系膜细胞ROS和p38MAPK的影响  

Effects of pioglitazone on high glucose mediated ROS and p38MAPK in cultured rat mesangial cells

作　　者：汪姗[1] 叶山东[1] 孙文佳[1] 刘皆[1] 胡圆圆[1] 

机构地区：[1]安徽医科大学附属省立医院内分泌科,安徽合肥230001

出　　处：《中国药理学通报》2014年第1期82-85,共4页Chinese Pharmacological Bulletin

基　　金：安徽省自然科学基金资助项目(No 11040606M161);安徽高校省级自然科学研究项目(KJ2011A157)

摘　　要：目的观察吡格列酮(PIO)对高糖培养的肾小球系膜细胞(MCs)p38丝裂原活化蛋白激酶(p38MAPK)表达和活性氧(ROS)水平的影响,探讨PIO肾保护作用及机制。方法体外培养MCs,随机分为正常对照组(NG组)、高糖(HG组)及高糖+不同浓度吡格列酮组。应用流式细胞术检测细胞ROS水平,半定量RT-PCR测定MCs p38MAPK mRNA表达情况。结果与NG组比较,HG组细胞内ROS水平明显增加,p38MAPK mRNA表达增多(P<0.01);各PIO干预组上述变化明显受抑制(P<0.01或P<0.05),且呈剂量依赖性。结论吡格列酮可拮抗高糖诱导的MCs内ROS和p38MAPK高表达。Aim To observe the effects of pioglitazone on the level of intracellular reactive oxygen species （ROS） and the expression protein kinase （p38MAPK） of p38 mitogen-activated in cuhured rat mesangial cells（MCs） and explore its renoprotective mechanism. Methods MCs were cultured in the medium with nor- glucose concentration （group NG）, high glucose concentration （ group HG ） and different concentrations of pioglitazone. The level of ROS was determined by flow cytometry. The expression of p38MAPK mRNA was detected by semiquantitative RT-PCR. Results Compared with group NG, ROS production and p38MAPK mRNA expression significantly increased （P 〈 0. 01 ） in group HG. When treated with pioglita- zone, those changes mentioned above were improved in a concentration-dependent manner （ P 〈 0. 05 or P 〈 0.01 ）. Conclusion Pioglitazone can suppress high glucose-induced ROS production and p38MAPK ex- pression in a concentration-dependent manner in MCs.

关 键 词：P38MAPK 活性氧 氧化应激 吡格列酮 系膜细胞 糖尿病肾病 

分 类 号：R-332[医药卫生] R322.61