猴脑纹状体边缘区脑啡肽样免疫反应阳性突触的超微结构研究  

Ultrastructural characteristics of synapses consisted of M-enkephalin immunoreactive terminals in the marginal division of the monkey striatum

作　　者：王斌[1] 毛姣玉[1] 马林[2] 路蒙莎 吴升 陈衍晨[1] 舒斯云[1] 

机构地区：[1]南方医科大学珠江医院儿科中心,广州510282 [2]中国人民解放军总医院放射诊断科,北京100853 [3]南方医科大学南方医院电镜教研室,广州510515 [4]杭州市陆军疗养院,310007

出　　处：《中华神经医学杂志》2014年第1期45-49,共5页Chinese Journal of Neuromedicine

基　　金：国家自然科学基金（81271524、81371514）

摘　　要：目的观察猴脑边缘区甲硫氨酸-脑啡肽（MET-ENK）阳性免疫反应神经终末突触的超微结构，为进一步探索其学习记忆功能提供超微结构基础。方法选取健康成年雄性恒河猴6只，常规灌注固定和取脑，冰冻切片后行免疫组织化学染色检测纹状体MET-ENK的表达．取边缘区内免疫组化染色阳性反应区进行超微切片，电镜下观察边缘区MET-ENK阳性反应神经终末突触的超微结构。结果免疫组织化学染色显示在壳核和苍白球之间有一条密集排列的MET-ENK免疫反应阳性细胞带，即纹状体边缘区；电镜下可见边缘区神经元为长梭形或纺锤形，细胞器较少。MET-ENK阳性反应神经终末突触主要有5个类型：轴-树突触、轴-棘突触、轴-体突触、轴-轴突触以及多种类型的复合型突触。结论猴脑纹状体边缘区MET-ENK阳性免疫反应神经终末突触结构具有多样性和复杂性，明显不同于纹状体其他部分。Objective Marginal division （MrD） of striatum is a universal structure in the mammalian brain, and it plays an critical role in learning and memory. In the present study, we try to investigate the synaptic ultrastructure of Methionine enkephalin （MET-ENK） immunoreacted fibers connected with neurons in the marginal division of the striatum in the monkey brains to explore the ultrastructural basis of the mechanism of learning and memory function in MrD. Methods Six male monkeys （macaque） were perfused with paraformaldehyde through heart to fix the brain and the brains were sectioned by a cryostat. Sections of the brains were performed immunohistochemical staining to detect the MET-ENK expression in the striatum; the areas with positive immumohistochemical staining was performed ultrastructural observation for morphological characteristics of the MET-ENK synapses in the MrD. Results Immunohistochemistry staining showed a dense arrangement of MET-ENK immunoreactive cells between the putamen and globus pallidus. Five major types of MET-ENK synapses were identified in the MrD： the axo-dendritic synapses, the axo-spinous synapses, the axo-somatic synapses, the axo-axonic synapses and the compound synapses. Conclusion The MET-ENK synapses in the MrD are diverse and complex, and can be distinguished from the rest of the striatum.

关 键 词：基底节 甲硫氨酸脑啡肽 学习和记忆 超微结构 电镜 

分 类 号：R-332[医药卫生]