肿瘤坏死因子α诱导蛋白2在过敏性鼻炎小鼠模型中的表达及与血管生成的关系  

Expression of tumor necrosis factor-α-inducible protein-2 in a mouse model of allergic rhinitis and its relationship with angiogenesis

作　　者：王敏[1,2] 杨军[1,2] 尚军[3] 张罗[1,2] 

机构地区：[1]首都医科大学附属北京同仁医院耳鼻咽喉头颈外科,北京100730 [2]北京市耳鼻咽喉科研究所基础室,耳鼻咽喉头颈科学教育部重点实验室,北京100005 [3]首都医科大学附属北京同仁医院中心实验室,北京100730

出　　处：《首都医科大学学报》2013年第6期790-794,共5页Journal of Capital Medical University

基　　金：国家杰出青年科学基金(81025007);首都医科大学附属北京同仁医院科研基金(2012-YJJ-002)~~

摘　　要：目的观察过敏性鼻炎中肿瘤坏死因子α诱导蛋白2(tumor necrosis factor-α-inducible protein-2,TNFAIP2)的表达及与血管生成的关系。方法建立卵清蛋白(ovalbumin,OVA)诱导的过敏性鼻炎小鼠模型,检测TNFAIP2和血管内皮细胞标记(CD31)的表达,并分析相关性。结果成功建立OVA诱导的过敏性鼻炎小鼠模型,表现为小鼠挠鼻次数、血清OVA特异性IgE浓度、鼻黏膜局部嗜酸细胞和肥大细胞的聚集均显著高于正常对照。TNFAIP2 mRNA和蛋白及CD31mRNA在过敏性鼻炎小鼠鼻黏膜局部的表达均明显高于正常对照。TNFAIP2和CD31mRNA的表达存在正相关。结论 TNFAIP2参与了过敏性鼻炎的发生,且可能与血管生成有关。Objective To investigate the expression of tumor necrosis factor-a-inducible protein-2 （TNFAIP2） in allergic rhinitis and its relationship with angiogenesis. Methods Allergic rhinitis mouse model was induced by ovalbumin（OVA）, expression of TNFAIP2 and CD3 l （the marker of endothelial cells） in nasal mucosa was measured and correlation analysis was done. Results The number of nasal rubbing, serum OVA-specific IgE levels, and nasal mucosal eosinophil and mast cell infiltration were significantly higher in allergic rhinitis mouse model than in the control mice. Compared to the control mice, expression of TNFAIP2 mRNA and protein and CD31 mRNA in allergic rhinitis mouse model increased significantly. TNFAIP2 expression positively correlated with CD31. Conclusion TNFAIP2 may be involved in the development of allergic rhinitis via regulating the angiogenesis.

关 键 词：肿瘤坏死因子α诱导蛋白2 血管生成 过敏性鼻炎 

分 类 号：R392.8[医药卫生—免疫学]