解聚素样金属蛋白酶10在小鼠颅底软骨联合发育过程中的时空表达  

Spatiotemporal expression of ADAM10 during cranial base suture development

作　　者：傅润卿[1] 谭宇[1] 杨秩[1] 房兵[1] 

机构地区：[1]上海交通大学医学院附属第九人民医院·口腔医学院口腔颅颌面科,上海市口腔医学重点实验室,上海200011

出　　处：《上海口腔医学》2013年第6期601-606,共6页Shanghai Journal of Stomatology

基　　金：国家自然科学基金(10972142);国家自然科学基金青年项目(81000451);上海市卫生局青年科研项目(2010y142);上海市教育委员会优秀青年教师项目[沪教委人(2010)83];上海市科学技术委员会重点研究项目(12JC1405700);上海市教育委员会创新团队项目~~

摘　　要：目的:研究解聚素样金属蛋白酶10(a disintegrin and metalloproteinase 10,ADAM10)在小鼠颅底软骨联合发育过程中的表达变化,为进一步探索ADAM10在颅底发育中的作用奠定基础。方法:对不同发育阶段的小鼠进行组织学和生化学研究,观察并分析ADAM10在小鼠颅底软骨联合发育过程中的时空表达。结果:小鼠颅底的蝶枕、蝶筛联合组织结构基本相似,分为静息区、增殖区、肥大区;联合区ADAM10表达广泛,在肥大区的表达高于静息区和增殖区;小鼠胚胎及幼年期ADAM10表达量高,到成年期表达量明显下降;ATDC5细胞中,ADAM10表达于胞质包膜区,部分信号与核周的高尔基体共标。结论:ADAM10在小鼠颅底软骨联合发育过程中具有时空规律性,可能参与颅底软骨内成骨过程。PURPOSE： To explore the expression of a disintegrin and metalloproteinase 10 （ADAM10） during mouse＇s development of cranial base synchondrosis. METHODS： Histological and biochemical method including alcian blue- alizarin red staining, IHC, ICC and Western blot were applied to observe and analyze the expression of ADAM10 in mouse cranial base. RESULTS： The spheno-occipital synchondrosis and spheno-ethmoid synchondrosis were of similar histological structure, including resting zone, proliferative zone and hypertrophic zone. ADAM10 was expressed widely in synehondrosis,espeeially in the hypertrophic zone. During development of cranial base, ADAM10 was highly expressed from embryo period to infantile period, while there was a huge decrease when it came to adulthood. As an excellent cell line to mimic chondrogenesis, ATDC5 expressed ADAM10 in cytoplasm, particularly around the nucleus. CONCLUSIONS： The expression of ADAM10 during mouse＇s development of cranial base synchondrosis is spatiotemprally different. Supported by National Natural Science Foundation of China （10972142）, National Science Foundation for Distinguished Young Scholars of China （81000451）,Science Foundation for Young Scholars of Shanghai Municipal Public Health Bureau （2010y142）, Foundation for Distinguished Youth Teachers of Shanghai Municipal Commission of Education [（2010）83], Key Research Project of Science and Technology Commission of Shanghai Municipality （12JC1405700） and InnovationTeam Project of Shanghai Municipal Commission of Education.

关 键 词：解聚素样金属基质蛋白酶10 颅底软骨联合 时空表达 

分 类 号：R782[医药卫生—口腔医学]