5种牙本质黏结剂对人牙周膜细胞毒性的比较  

A comparative study of the cytotoxicity of five dental bonding agents to human periodontal ligament cells

作　　者：李俊亮[1] 王文梅[1] 葛久禹[1] 季骏[2] 王天丛[2] 

机构地区：[1]南京大学医学院附属口腔医院牙体牙髓病科 [2]南京大学医学院附属口腔医院口腔正畸科,南京大学口腔医学院中心实验室,江苏南京210008

出　　处：《上海口腔医学》2013年第6期628-633,共6页Shanghai Journal of Stomatology

基　　金：江苏省医学领军人才与创新团队项目(LJ201110);江苏省自然科学基金(SBK201340904)~~

摘　　要：目的:比较5种牙本质黏结剂对人牙周膜细胞的毒性作用。方法:原代培养人牙周膜细胞,采用四甲基偶氮唑盐比色法(MTT)和浸提液法,观察5种牙本质黏结剂(Super Bond C&B、Clearfil SE Bond、G-Bond、Single Bond2、Adper Easy One)不同体积分数浸提液(100%、50%、25%)作用不同时间(24 h、48 h、72 h)对人牙周膜细胞的毒性作用。采用SPSS19.0软件包对数据进行统计学分析。结果:5种牙本质黏结剂对人牙周膜细胞的细胞毒性Super Bond<G-Bond<Clearfil SE Bond<Adper Easy One<Single Bond2。作用时间和浓度的改变对其细胞毒性有一定影响,各实验组细胞毒性25%<50%<100%;细胞毒性24 h<48 h<72 h,24 h与48 h有显著差异(P<0.05),48 h与72 h无显著差异(P>0.05)。结论:5种牙本质黏结剂细胞毒性程度不同,Super Bond C&B、G-Bond和Clearfil SE Bond的细胞毒性均较轻微,Single Bond2的细胞毒性较强。PURPOSE： To compare the cytotoxicity of 5 different dental bonding agents to human periodontal ligament cells（HPDLCs）. METHODS： Primary cultured HPDLCs were exposed to different concentrations （100%,50% and 25%）of Super Bond, Clearfil SE Bond, G-Bond, Single Bond2, and Adper Easy One for different times （24 h, 48 h and 72 h）. Cytotoxicity of 5 different agents to HPDLCs were observed by MYl~ method. The data was analyzed using SPSS 19.0 software package. RESULTS： Cytotoxicity of 5 dentin bonding agents to HPDLCs were Super Bond〈Clearfil SE Bond〈G- Bond〈Adper Easy One〈Single Bond2. Cytotoxicity was affected by the exposure time and concentration. Cytotoxicity effect was 25%〈50%〈100% and 24 h〈48 h〈72 h. Significant difference was found between 24h and 48h （P〈0.05）but no significant difference was found between 48h and 72h （P〉0.05）. CONCLUSIONS： Cytotoxicity of 5 dentin bonding agents are different. Cytotoxicity of Super-bond C＆B, G-Bond and Clearfil SE Bond are weak, while cytotoxicity of Single Bond2 is the strongest among the 5 agents. Supported by Medical Leading Talents and Innovation Team Project of Jiangsu Province （LJ201110） and Natural Science Foundation of Jiangsu Province （SBK201340904）.

关 键 词：牙本质黏结剂 人牙周膜细胞 细胞毒性 

分 类 号：R783.1[医药卫生—口腔医学]