分泌卷曲相关蛋白在甲状腺相关眼病发病中的作用研究  

Role of secreted frizzled-related protein in pathogenesis of thyroid-associated ophthalmopathy

作　　者：盛海斌[1,2] 王计秋[3] 蔡昌枰[1] 焦秦[4] 程瑜[4] 

机构地区：[1]上海交通大学医学院附属瑞金医院耳鼻喉科,上海200025 [2]复旦大学附属眼耳鼻喉科医院,博士研究生上海200031 [3]上海交通大学医学院附属瑞金医院内分泌科,上海200025 [4]上海交通大学医学院附属瑞金医院眼科,上海200025

出　　处：《内科理论与实践》2013年第6期406-409,共4页Journal of Internal Medicine Concepts & Practice

基　　金：上海市卫生局面上项目(项目编号:2010279);上海市级医院新兴前沿技术项目(项目编号:SHDC12013121)

摘　　要：目的：研究分泌卷曲相关蛋白（SFRP）在甲状腺相关眼病（TAO）发病中的作用。方法：收集9例TAO并行鼻内镜下眶减压术患者（病变组）眶脂肪组织及7例眼科由于外伤行眼球摘除术或眼外肌矫形术患者（对照组）术中切除的正常眶脂肪组织．应用实时荧光定量PCR技术检测病变组与对照组眶脂肪组织中SFRPl～5以及过氧化物酶体增殖物激活受体γ（PPAR-γ）mRNA的表达，应用蛋白质印迹技术检测病变组与对照组眶脂肪组织中SFRP2和PPAR-1的蛋白表达情况．分析其差异。结果：病变组眶脂肪组织中SFRP2的mRNA表达水平高于对照组（P〈0．05），SFRPl、SFRP3、SFRP4、SFRP5的mRNA表达水平2组间差异无统计学意义。病变组眶脂肪组织中PPAR-1的mRNA表达水平高于对照组（P〈0．01）。病变组眶脂肪组织中SFRP2及PPAR-γ的蛋白水平均较对照组明显升高（均P〈0．01）。结论：SFRP2可能在TAO患者发病中起一定作用．并可能是通过PPAR一叫的升高引起眶脂肪增生。Objective To study the role of secreted frizzled-related protein （SFRP） in pathogenesis of thyroid-associ- ated ophthalmopathy （TAO）. Methods Orbital adipose tissue was collected from 9 patients with TAO undertaken endo- scopic orbital decompression （TAO group） and 7 patients undertaken ophthalmectomy or extraocular muscle surgery（control group）, and real time quantitative PCR was used to analyze the gene expression of SFRP 1-5 and peroxisome proliferator- activated receptor（PPAR）-γ in the orbital adipose tissue of patients with TAO and controls, and Western blot was used to analyze the protein expressions of SFRP 2 and PPAR-γ. Results The mRNA level of SFRP2 in TAO group orbital adipose tissue was higher than that in control group（P〈0.05）. The mRNA levels of SFRP1, SFRP3, SFRP4, SFRP5 in TAO group orbital adipose tissue were not different from that in control group （P〉O.05）. The mRNA level of PPAR-~/in TAO group orbital adipose tissue was significantly higher than that in control group （P〈0.01）. Levels of SFRP2 and PPAR-~ protein were both higher in TAO group orbital adipose tissue than that in control group. Conclusions SFRP2 probably takes part in the pathogenesis of TAO, it may increase the level of PPAR-γ in TAO orbital adipose tissue and thereby increasing the amount of adipose tissue.

关 键 词：甲状腺相关眼病 分泌卷曲相关蛋白 过氧化物酶体增殖物激活受体 脂肪细胞 

分 类 号：R771.3[医药卫生—眼科]