Sigmoidal拟合曲线法研究银杏叶类脂轻馏分的抗氧化性作用  

Antioxidant Effect of Light Distillates Separated by Molecular Distillation from Lipids of Ginkgo Biloba L. Leaves Using Sigmoidal Curve Fitting Method

作　　者：陶冉[1,2,3,4] 王成章[1,2,3,4,5] 孔振武[1,2,3,4,5] 

机构地区：[1]中国林业科学研究院林产化学工业研究所 [2]生物质化学利用国家工程实验室 [3]国家林业局林产化学工程重点开放性实验室 [4]江苏省生物质能源与材料重点实验室,江苏南京210042 [5]中国林业科学研究院林业新技术研究所,北京100091

出　　处：《林产化学与工业》2013年第6期14-18,共5页Chemistry and Industry of Forest Products

基　　金：“十二五”国家科技支撑计划资助(2011BAD33B02);江苏省产学研前瞻性联合研究项目(BY2012042)

摘　　要：通过石油醚提取、皂化、分子蒸馏以及硅胶柱色谱分离等方法得到了7组银杏叶类脂不同极性轻馏分,用4种抗氧化测定方法(DPPH、ABTS、超氧阴离子和羟自由基法),结合Sigmoidal拟合曲线法求银杏叶类脂不同极性轻馏分的半数清除浓度(IC50),来评价银杏叶类脂轻馏分的抗氧化作用。结果显示,银杏叶类脂轻馏分具有一定抗氧化作用,其中馏分2(95%石油醚/5%乙酸乙酯部分)在各馏分组中抗氧化性最强,IC50分别为(51.0±0.061),(51.3±0.042),(56.9±0.045)和(124.4±0.037)mg/L;所有馏分样品中偏离最大的清除率实验值与清除率为50%的相对偏差为1.4%,表明Sigmoidal拟合曲线法求银杏叶类脂轻馏分不同极性馏分的IC50是合理的。The fractions separated from light distillates of ginkgo lipids were prepared by extraction with petroleum ether,saponification,molecular distillation and silica gelcolumn chromatographic isolation.Four methods （DPPH,ABTS,superoxide anion and hydroxy radical） with Sigmoidal curve fitting method arriving at respective IC50 values were used to determine the antioxidant effect of the seven fractions.The results showed that light distillates of ginkgo lipids had antioxidant effect and the fraction 2 （95％ petroleum ether/5％ acetic ether） had the highest antioxidant effect in all tested fractions.IC50 values were （51.0 ± 0.061）,（51.3 ± 0.042）,（56.9 ± 0.045） and （124.4 ± 0.037） mg/L.The relative deviation between experimental scavenging rate and scavenging rate （50％） of the fraction having maximum deviation in all fractions was 1.4％.Consequently,it is reasonable that the IC50 values of different polar fractions of light distillates separated from ginkgo lipids are generated with Sigmoidal curve fitting method.

关 键 词：银杏 Sigmoidal拟合曲线法 类脂 抗氧化性 

分 类 号：TQ35[化学工程]