西安地区宫颈癌组织中高危型人乳头瘤病毒型别谱初步分析  

The preliminary analysis of the prevalence of HPV genotype distribution in cervical cancer in Xi'an

作　　者：许锋[1] 杨波[1] 赵复联 王煜[1] 盛慧萍[1] 谢丽[1] 唐先梅[1] 杨筱凤[1] 

机构地区：[1]西安交通大学第一附属医院转化医学中心,陕西西安710061

出　　处：《西北大学学报（自然科学版）》2013年第6期924-926,共3页Journal of Northwest University（Natural Science Edition）

基　　金：教育部博士点基金资助项目(20070698097)

摘　　要：分析西安地区宫颈癌组织中高危HPV病毒谱的分布以及宫颈癌组织中多重HPV感染的状况。从149例经病理组织学证实的浸润性宫颈癌组织样本中提取基因组DNA,设计8种高危HPV型别特异型引物,常规PCR扩增HPV E6 DNA片段,琼脂糖凝胶电泳检测,并进行统计分析。宫颈癌组织样本中HPVDNA检出率为87.3%,其中39.6%可检出一种型别以上的HPVDNA。HPV16型最为常见(73.2%),其余依次为HPV18,58,52,33,45,31(41.6%,11.4%,5.4%,2.7%,2.0%,1.3%,0),单一感染者47.7%,合并感染者39.6%。西安地区宫颈癌组织中HPV16,18,58,52为主要流行株。To analyse the HPV genotype profile and the presence of multiple HPV infections in invasive cervical cancer in Xi＇an. 149 cases of invasive cervical cancer samples with histologically confirmation were cllected from the First afffiliated hospital, the specimens were routine formalin fixed and paraffin embedded. The samples were cut and the genome DNA were abstracted with the commercial kits. The HPV genotyping was performed by routine PCR amplification technique with the type specific primers which differentiates 8 high risk HPV genotypes. HPV was detected in 87.3% of the samples, of whom 39.6% were infected with more than one HPV genotype. HPV16 was most common, being detected in 73.2% of all cases, followed by HPV18, 58, 52, 33, 45 and 31 （41.6%, 11.4%, 5.4%, 2.7%, 2.0%, 1.3% ,0）. HPV16,18,58 and 52 accounted for the most prevalent HPV geno- types.

关 键 词：西安地区 宫颈癌 高危型HPV 型别 

分 类 号：R737.33[医药卫生—肿瘤]