白花丹醌—单壁碳纳米管偶联物的制备及其对人肝癌Bel-7402细胞的抑制作用  

SYNTHESIS OF PLUMBAGIN-SINGLE WALLED CARBON NANOTUBE CONJUGATE AND ITS INHIBITORY EFFECT ON HUMAN HEPATOCARCINOMA BEL-7402 CELLS

作　　者：赖泽锋[1] 钟全强[1] 秦悦[1] 李苏宁[1] 冯建海[1] 赵雅婷[1] 刘华钢[1] 

机构地区：[1]广西医科大学药学院,南宁530021

出　　处：《广西医科大学学报》2013年第6期829-833,共5页Journal of Guangxi Medical University

基　　金：国家自然科学基金资助项目(No.81260657);广西自然科学基金资助项目(No.2012GXNSFBA053112);广西教育厅科研基金资助项目(No.200103YB024)

摘　　要：目的:制备白花丹醌—单壁碳纳米管(plumbagin-single walled carbon nanotubes conjugate,PLB-SWNT)偶联物,考察其在水溶液中的分散性和体外对人肝癌Bel-7402细胞的抑制活性。方法:以聚乙二醇(polyethylene glycol,PEG)作为偶联剂,通过酰胺化和酯化反应将PLB与SWNT偶联,制备PLB-SWNT偶联物,并利用傅里叶转换红外光谱仪和透射电子显微镜(TEM)考察和分析所制备得到的偶联物的组成、结构和形貌,以紫外—可见分光光度法(UV-Vis)测定PLB-SWNT偶联物的PLB含量,并以MTT比色法、HE染色法和TEM等方法对该偶联物体外抑制Bel-7402活性进行初步研究。结果:①制备得到在水溶液中分散性较好的PLB-SWNT偶联物;②PLB-SWNT偶联物能够抑制人肝癌Bel-7402细胞的增殖。结论:①以PEG和PLB对SWNT进行共价修饰,可以制备分散性良好的PLB-SWNT偶联物;②PLB-SWNT偶联物在体外对人肝癌Bel-7402细胞的增殖抑制效果显著。Objective：To synthesize plumbagin-single walled carbon nanotube conjugate （PLB-SWNT）, and to investigate its dispersibility in water and inhibitiory effect on human hepatocarcinoma Bei-7402 cells in vitro. Methods： Conjugating plumbagin and single walled carbon nanotubes by amidation and esterification reaction was used by using polyethylene glycol （PEG） as coupling agent. Components, structure and mor- phology of the prepared conjugate were investigated and analyzed by using Fourier transform infrared spec- troscopy （FT-IR） and transmission electron microscope （TEM）. The inhibitory effect of conjugates on Bel- 7402 was tested in vitro by MTT assay, HE staining and TEM observation. Results： The as-prepared PLB- SWNT conjugate exhibited high water dispersibility. PLB-SWNT conjugate could inhibit the proliferation of hepatocarcinoma Bel-7402 cells. Conclusion： The highly dispersive PLB-SWNT conjugate in water could be obtained after functioning SWNTs by using PEG and PLB. The inhibitory effect of PLB-PEG-SWNTs on the proliferation of tumor cells in vitro was remarkable.

关 键 词：白花丹醌 单壁碳纳米管 偶联物 药物载体 肝癌BEL-7402细胞 抑制作用 

分 类 号：R735.7[医药卫生—肿瘤]