小胶质细胞在视网膜母细胞瘤中的活化及分布研究  

Activation and distribution of microglia in retinoblastoma

作　　者：陈雪[1] 李海平[2] 张培[2] 曹安民 

机构地区：[1]北京和睦家医院眼科,北京市100015 [2]北京大学第三医院眼科,北京市100191 [3]霍普金斯大学医学院威尔玛眼科研究所,美国巴尔的摩21287

出　　处：《眼科新进展》2014年第1期29-33,共5页Recent Advances in Ophthalmology

摘　　要：目的对人视网膜母细胞瘤标本中小胶质细胞的活跃性及其与肿瘤分化程度、肿瘤侵袭性的相关性进行分析,了解小胶质细胞在视网膜母细胞瘤中的活化程度和分布特点。方法共收集32例33眼人视网膜母细胞瘤病例标本,总结其临床资料及肿瘤细胞分化特点。以MHCII作为小胶质细胞标志物、MAP-2为肿瘤细胞标志物进行免疫组织化氉学染色和免疫荧光双标染色。应用SPSS软件对数据进行χ2检验,分析小胶质细胞细胞分布与肿瘤分化情况及肿瘤侵犯情况的关系。结果在人视网膜母细胞瘤标本中,MHCII阳性细胞计数与肿瘤分化程度相关(OR=4.8,P=0.041)。在低分化肿瘤中,小胶质细胞数量相对较少;而中高分化肿瘤中,小胶质细胞数量较多。MHCII阳性细胞计数与肿瘤侵袭性及前期放化疗情况无相关性。表达MAP-2的肿瘤细胞不表达MHCII。结论小胶质细胞在视网膜母细胞瘤中是活化的,分布特征与肿瘤细胞的分化程度密切相关,其生物学意义和细胞机制有待于进一步研究来阐释。Objective To analyze the activation of retinal microglia in human retinoblastoma and its correlation with retinoblastoma differentiation and tumor inva sion, and investigate its active degree and distribution. Methods Thirty-three cases of retinoblastoma （33 eyes） were examined, and the clinical and histology data were sum marized. Paraffin embedded tumor sections were stained with MHCII as marker of acti vated microglia and MAP-2 as marker of tumor cells in immunohistochemistry staining. The correlation of immunoreactivity with degree of tumor differentiation and tumor in vasion of choroid and optic nerve were analyzed. Chisquare analysis was carried out with SPSS software. Results Positive MHCII cells in human retinoblastoma were closely related with differentiation of tumors （OR = 4. 8 ）. In poorly differentiated tumor,proportion of high MHCII expression was 38.46% , while, in moderate and well differentiated tumor,the proportion of high MHCII expression was 75.00% , there was statistical difference （P 〈 0.05 ）. The positive MHCII cell was not statistically associated with invasion of tumor or previous chemo/radio therapy. Positive MAP-2 tumor cells did not express MHCII. Conclusion The microglia is activated in retinoblastoma,its dis tribution is closely related with differentiation of tumor, and the involved biological sig nificance and pathways need further exploration.

关 键 词：小胶质细胞 视网膜母细胞瘤 肿瘤分化 

分 类 号：R739.7[医药卫生—肿瘤]