TAM和MA序贯或联合对乳腺癌Bcap-37细胞生长抑制作用的研究  

STUDIES ON INHIBITORY EFFECT OF TAM AND MA SEQUENTIAL OR COMBINATION ON CULTURED HUMAN BREAST CANCER BCAP 37 CELL IN VITRO

作　　者：王晓稼[1] 郑树[1] 吴金民[2] 程兆明[3] 

机构地区：[1]浙江大学肿瘤研究所,浙江杭州310009 [2]浙江大学逸夫医院,浙江杭州310016 [3]暨南大学医学院,广东广州515000

出　　处：《肿瘤研究与临床》2000年第6期378-380,共3页Cancer Research and Clinic

摘　　要：目的 :通过体外 MA和 TAM单药、不同序贯以及联合对乳腺癌 Bcap- 37细胞生长抑制作用研究 ,以指导临床选择合理的 TAM/ MA治疗方案。方法 :模仿临床而设计了 3个 TAM/ MA联合及两者单药的治疗方案 ,观察其体外对乳腺癌 Bcap- 37细胞的生长抑制作用 ,以 MTT法测定其细胞存活率。结果 :在低浓度时 TAM和MA体外对 Bcap- 37细胞的生长抑制作用相近 ,而 2 .5 mg/ L浓度以后 TAM的生长抑制作用显著高于 MA(P<0 .0 5 )。TAM→ MA序贯各浓度的肿瘤细胞生长抑制作用较 MA→ TAM序贯和 TAM+ MA联合为强 (P<0 .0 5 )。TAM+ MA和 MA→ TAM相比 ,在低浓度 (0 .2 5 m g/ L～ 2 .5 m g/ L)时两者肿瘤生长抑制作用相近。当 MA浓度为5 .0 m g/ L 和 7.5 m g/ L 时 ,联合用药组生长抑制作用更强 (分别为 P=0 .0 5和 P<0 .0 5 )。结论 :在 TAM与 MA不同联合治疗方案中以 TAM→Objective:To study the inhibitory effect of TAM or MA single,and TAM/MA sequential or combination on cultured human breast cancer cell line (Bcap 37 cell line)in vitro,in order to guide us on selecting the best regimen of TAM MA sequential or combination.Methods:Three regimens of TAM/MA combination therapy and single drug(TAM or MA)were designed and their inhibitory effects to human breast cancer cell line (Bcap 37 cell line) in vitro(MTT assay)were observed.Results:(1)The inhibition of TAM and MA single to Bcap 37 cell is similar in low concentration(0.25 mg/L to 2.5 mg/L).From 2.5 mg/L to 7.5 mg/L,the inhibitory effects of TAM was greater than that of MA significantly( P <0.05).(2)The inhibitory effect of TAM→MA sequential was greater than the MA→TAM sequential or TAM+MA combination in all concentration( P <0.05).In low concentration,the inhibition of TAM+MA and MA→TAM sequential was similar,but in high concentration,the former became greater remarkably( P =0.05 and P <0.05,respectively).Conclusion:In the TAM and MA combination therapy regimens,the TAM→MA sequential or the TAM/MA combination therapy is the best choice.

关 键 词：三苯氧胺 甲地孕酮 乳腺癌 Bcap-37细胞 体外 

分 类 号：R737.9[医药卫生—肿瘤]