p53在不同病变宫颈组织中的突变及其意义  

STUDY OF p53 GENE MUTATIONS IN CERVICAL LESIONS

作　　者：李惠芳[1] 常艳丽[1] 

机构地区：[1]山西长治医学院,山西长治046000

出　　处：《肿瘤研究与临床》2000年第6期399-401,共3页Cancer Research and Clinic

基　　金：山西省自然科学基金资助项目(94036)

摘　　要：目的 :探讨抑癌基因 p5 3外显子 7- 8突变在宫颈癌发生中的作用和意义。方法 :聚合酶链反应(PCR)、单链构象多态性 (SSCP)分析。结果 :15 0例不同病变宫颈组织中均未见 p5 3外显子 7- 8缺失 ;可见 p5 3外显子 7- 8突变在宫颈癌、宫颈不典型增生、宫颈息肉组织中分别为 14.2 9% (7/ 49)、16 % (8/ 5 0 )、3.85 % (1/ 2 6 ) ;宫颈慢性炎症组织中未见突变。p5 3外显子 7- 8突变在宫颈癌、宫颈不典型增生和宫颈息肉之间无显著差异 (χ2 =0 .36 1,P>0 .5 ) ;且与宫颈癌的分化程度、浸润深度及宫颈不典型增生的病理分级无显著相关性 (r<0 .997,P>0 .0 5 )。结论 :在宫颈癌发生中 p5 3外显子 7-Objective:To investigate the role and significance of p53 (exon 7 8) mutations in the oncogenesis of cervical cancer.Methods:Polymerase chain reaction (PCR) and single strand conformation polymorphism(SSCP) was used.Results:The defects of p53 (exon 7 8) were not found in 150 cervical lesions,but p53 (exon 7 8) mutations were respectively detected in 7 of 49 (14.29%) cervical cancer,in 8 of 50 (16%) cervical dysplasia and in 1 of 26 (3.85%) cervical polyp,and in zero of 25 chronic cervicitis.There was no significant difference of p53 mutations among cervical cancer,cervical dysplasia,cervical ployp (χ 2=0.361, P >0.5),and p53 mutations had no relation with the differentiation and invasive degree of cervical cancer and the pathological degree of cervical dysplasia ( r <0.997, P >0.05).Conclusion:p53 (exon 7 8) mutations is an important and early factor in the oncogenesis of cervical cancer.

关 键 词：宫颈肿瘤 宫颈不典型增生 抑癌基因P53 基因突变 

分 类 号：R730.2[医药卫生—肿瘤]