岩白菜素抗高尿酸血症的活性及机制研究  

Anti-hyperuricemic activity of bergenin and its mechanism

作　　者：周宏星[1] 陈玉胜[2] 

机构地区：[1]江苏省丹阳市人民医院药剂科,丹阳212300 [2]南京晓庄学院生物化工与环境工程学院食品教研室,南京211171

出　　处：《安徽医科大学学报》2014年第1期63-67,共5页Acta Universitatis Medicinalis Anhui

基　　金：江苏省高等学校大学生实践创新项目(编号:2161211)

摘　　要：目的在氧嗪酸钾盐诱导小鼠产生高尿酸血症模型的基础上,探讨岩白菜素的抗高尿酸活性。方法将60只昆明种雄性小鼠随机均分为6组:正常对照组、高尿酸血症模型组、岩白菜素(20、40、60 mg/kg)组、别嘌呤醇(5 mg/kg)组。每天灌胃氧嗪酸钾盐(250 mg/kg)1 h后再灌胃给药,连续1周。分别测定血清中尿酸、肌酐和尿素氮水平,尿液中尿酸和肌酐水平,以及肾脏尿酸转运子1(mURAT1)和葡萄糖转运子9(mGLUT9)的mRNA和蛋白表达水平。结果与高尿酸血症模型组比较,岩白菜素显著降低高尿酸血症小鼠血清中尿酸、肌酐和尿素氮水平,提高24 h尿液尿酸和肌酐排泄量以及尿酸排泄分数,显著下调高尿酸血症小鼠肾脏mURAT1和mGLUT9的mRNA和蛋白表达水平。结论岩白菜素通过促进高尿酸血症小鼠肾脏尿酸排泄作用及逆转尿酸转运体的过度表达,从而体现抗高尿酸活性。Objective To explore the anti-hypemricemia activity of bergenin in the model of hyperuricemie mice induced by potassium oxonate. Methods 60 Kunming male mice were divided randomly into six groups, which were normal control group; hyperuricemic model group; and hyperuricemic groups with 20, 40, 60 mg/kg bergenin, and 5 mg/kg allopurinol. Mice were orally administered once daily with 250 mg/kg potassium oxonate for 7 continuous days to create the model, and then three doses of bergenin and allopurinol were orally initiated on the day 1 h after potassium oxonate was given, separately. Serum uric acid, creatinine and urea nitrogon levels, as well as urinary uric acid and creatinine levels were measured, mRNA and protein expression levels of mouse kidney urate transporter 1 （ URAT1 ）, and glucose transporter 9 （ GLUT9 ） were also determined. Results Compared with hyperuricemic model group, bergenin significantly reduced serum uric acid, creatinine and urea nitrogon levels, increased 24 h uric acid and creatinine excretion, and fractional excretion of uric acid in hyperuricemic mice ; mRNA and protein levels of mURAT1 and mGLUT9 were also markedly down-regulated. Conclusion Anti-hyperuricemia effect of bergenin is attributed to the enhancement of uric acid excretion and reversion of mouse urate transporters over-expression.

关 键 词：岩白菜素 高尿酸血症 氧嗪酸钾盐 小鼠 

分 类 号：R965.1[医药卫生—药理学]