MMP-14与EMMPRIN在口腔鳞状细胞癌中的表达及临床意义  

Expression and significance of MMP-14 and EMMPRIN in human oral squamous cell carcinoma

作　　者：陶冶[1] 张令达[1] 

机构地区：[1]安徽医科大学第一附属医院口腔颌面外科,合肥2300222

出　　处：《安徽医科大学学报》2014年第1期81-84,共4页Acta Universitatis Medicinalis Anhui

摘　　要：目的探讨基质金属蛋白酶-14(MMP-14)和细胞外基质金属蛋白酶诱导因子(EMMPRIN)在口腔鳞状细胞癌(OSCC)中的表达及其临床意义,并对二者的相关性进行分析。方法应用逆转录-聚合酶链反应(RT-PCR)技术对43例手术切除的OSCC组织及11例口腔正常组织(NOT)中的MMP-14及EMMPRIN mRNA的表达进行检测。结果 MMP-14及EMMPRIN mRNA在OSCC组中的表达均高于NOT组(P<0.05),在有淋巴结转移组的表达均高于无淋巴结转移组(P<0.05),两者的表达均与肿瘤的病理分期、分化程度及淋巴结转移有关,与患者的性别和年龄无关。相关性分析显示MMP-14及EMMPRIN mRNA之间呈正相关(r=0.801,P<0.01)。结论 MMP-14和EMMPRIN基因的异常表达与OSCC的发生、发展和转移密切相关,联合检测MMP-14和EMMPRIN mRNA的表达状况可以为评价OSCC恶性程度及患者预后提供参考。Objective To investigate the expression and significance of MMP-14 and EMMPRIN and the correlation between them in oral squamous cell carcinoma （OSCC）. Methods The expression of MMP-14 and EMMPRIN mRNA in 43 cases of OSCC and 11 cases of normal oral tissue were detected by RT-PCR. Results The expression of MMP-14 and EMMPRIN mRNA in OSCC was significantly higher than those in normal oral tissue （ P 〈 0. 05 ）. Compared with those without lymph node metastasis in OSCC, the expression of MMP-14 and EMMPRIN mRNA with lymph node metastasis was significantly higher （ P 〈 0. 05 ）. Furthermore, the expression of MMP-14 and EMMPRIN was significantly associated with pathological stage and the lymph node metastasis, but neither of them was correlated with sex and age. Correlation analysis showed that MMP-14 expression level was positively related to EMMPRIN level（ r = 0. 801 ,P 〈 0. 01 ）. Conclusion The expression of MMP-14 and EMMPRIN in OSCC is significantly correlated with occurrence, progress and metastasis of OSCC. Therefore, the combination of detecting the expression of MMP-14 and EMMPRIN mRNA may evaluate the degree of malignancy and predict the prognosis of patients with OSCC.

关 键 词：口腔鳞状细胞癌 逆转录-聚合酶链反应 基质金 属蛋白酶-14 细胞外基质金属蛋白酶诱导因子 

分 类 号：R739.8[医药卫生—肿瘤]