炎症性肠病患者肠组织蛋白表达差异分析  

Analysis of differential expressed protein in the intestinal mucosa of patient with inflammatory bowel disease

作　　者：吴迎[1] 胡佳辰[1] 周瑞[1] 夏冰[1] 

机构地区：[1]武汉大学中南医院消化内科湖北省肠病医学临床研究中心肠病湖北省重点实验室,430071

出　　处：《中华消化杂志》2014年第1期45-48,共4页Chinese Journal of Digestion

基　　金：基金项目：国家自然科学基金（81070280,81270467）

摘　　要：目的应用细胞因子抗体芯片技术研究IBD患者肠组织差异表达的蛋白质，探讨筛选出的蛋白在IBD发病机制中可能涉及的作用。方法收集9例UC、9例CD和9例对照患者的肠组织标本，每组提取总蛋白后，采用RaybiotechL系列人抗体芯片分析各组的差异表达蛋白质谱。另收集9例UC，9例CD和9例对照患者肠组织标本，用Western印迹法对差异较大的蛋白质进行验证。两组间比较采用￡检验。结果与对照组相比，UC组共有263个细胞因子表达发生显著变化，CD组共有414个细胞因子表达发生显著变化。随后采用Western印迹法验证了单纯疱疹病毒进入介导子、白血病抑制因子和血小板因子4在IBD患者肠组织中均高表达，差异均有统计学意义（UC组，t=23．85、9．53、18．88，CD组t=13．54、16．65、13．67，P均〈0．01）。结论采用抗体芯片技术筛选出IBD患者肠组织细胞因子差异表达谱，有利于揭示IBD发病机制及发现新的分子标志物。Objective To investigate differential expressed protein in the intestinal mucosa of patients with inflammatory bowel disease （IBD） with antibody chips, and to explore the possible role of the screened proteins in pathogenesis of IBD. Methods The mucosa tissues of nine patients with ulcerative colitis （UC）, nine patients with Crohn^s disease （CD） and nine control individuals were collected. After total protein of each group was extracted, the differential expressed protein of each group was analyzed by Raybiotech L-series human cytokine antibody chips. The mucose tissues of other nine patients with UC, nine patients with CD and nine control individuals were collected, and were used to verify the greatly differential expressed proteins by Western blot. The t-test was performed to compare two groups. Results Compared with the control group, there was significantly difference in 263 cytokines of UC group, and 414 cytokines of CD group. And then the higher expressions of herpes virus entry mediator, leukemia inhibitory factor and platelet factor 4 in the mucosa tissues of IBD patients were confirmed by Western blot and the differences were statistically significant （UC： t= 23.85, 9.53, 18. 88; CD： t= 13.54, 16.65, 13.67, all P〈0.01）. Conclusion The screened differential expressed cytokines in the mucosa tissues of IBD patients by cytokine antibody chips could be helpful to reveal the pathogenesis of IBD and discover new molecular biomarkers.

关 键 词：炎性肠疾病 蛋白质阵列分析 细胞因子类 印迹法 蛋白质 

分 类 号：R574[医药卫生—消化系统]