ANO1对口腔鳞癌SCC-25细胞株的影响  

The Effect of ANO1 on SCC-25 Oral Cancer Cell Line

作　　者：李雅冬[1] 张劲松[1] 杨凯[1] 张福军[1] 陈睿[1] 陈丹[1] 

机构地区：[1]重庆医科大学附属第一医院口腔科,重庆400016

出　　处：《中国细胞生物学学报》2014年第1期86-92,共7页Chinese Journal of Cell Biology

基　　金：2012年重庆市科委自然科学基金计划资助项目(批准号:cstc2012jjA10039);重庆市卫生局2011年医学科研计划项目(面上项目)(批准号:2011-2-013);重庆市教委科学技术研究项目(批准号:KJ130318)资助的课题~~

摘　　要：采用免疫组化检测160例口腔鳞癌组织及相应正常组织的ANO1表达,并进行多项体外实验,以明确ANO1对SCC-25细胞迁移的影响。结果显示,正常组织中的ANO1阳性表达明显低于口腔鳞癌组织;有淋巴结转移的口腔鳞癌组织的ANO1阳性表达显著高于无转移的口腔鳞癌组织;多项体外实验表明,ANO1过表达有利于SCC-25细胞的迁移;尼氟灭酸能减缓细胞的迁移速度。综上所述,ANO1促进了口腔鳞癌患者体内的肿瘤转移,并增加了SCC-25细胞的体外移动、侵袭、伸展、剥脱能力,有望成为治疗口腔鳞癌的新的靶点。The specimens of 160 patients with oral squamous cell carcinoma were examined by immunohistochemistry. SCC-25 cell line was used to do many assays in vitro to find out how ANO1 affect on the migration of SCC-25 cells. ANO 1 protein expression was significantly higher in oral squamous cell carcinoma than those in normal oral tissue, and showed significant correlation with clinical stage of oral squamous cell carcinoma and lymph node metastasis. The results of cell detachment assay, spreading assay, adhesion assay, boyden chamber migration assay and in vitro wound healing experiments showed that ANO1 overexpression favored the migration of SCC-25 cells; Niflumic Acid blocking chloride channel experiments showed that Niflumic Acid decreased the migration speed of SCC-25 cell. In summary, ANO 1 may promote clinic metastasis of oral squamous cell carcinoma. ANO1 overexpression significantly increases the migration, invasion, spreading, detachment capacities of oral squamous cell carcinoma, and these biological characteristics are significant correlated with invasion and metastasis of the tumor cell. so ANO 1 could become the new gene treatment target of oral squamous cell carcinoma.

关 键 词：口腔鳞癌 氯离子通道 转移 

分 类 号：R739.8[医药卫生—肿瘤]