N^+离子束注入对玉米种子和幼苗生物学效应的影响  

Biological Effect of Maize Seeds by Different N^+ Ion Implantation

作　　者：黄洪云[1] 杜宁[1] 张敬东[1] 

机构地区：[1]唐山学院,河北省唐山063000

出　　处：《种子》2014年第1期37-39,共3页Seed

基　　金：2012年度唐山市科学技术研究与发展第二批指导计划项目(编号:12120204b)

摘　　要：能量为30 keV的N+离子束,照射剂量范围0.5×1017ion/cm2~4.0×1017ion/cm2,注入玉米品种辽单120种子,测定玉米种子电导率、MDA含量及玉米幼苗叶片脯氨酸、MDA含量和POD、SOD酶活,研究不同照射剂量对玉米种子和幼苗生物学效应的影响。结果表明,照射剂量为3.5×1017ion/cm2时,玉米种子电导率和幼苗叶片MDA含量均较低,对细胞膜的破坏性小;玉米种子可溶性蛋白质和幼苗叶片脯氨酸含量、POD和SOD酶活较高,抗逆能力强,因此是最佳N+离子束照射剂量。研究结果对于利用N+离子束进行玉米诱变育种具有一定的指导作用。Maize seeds of Liaodan 120 were implanted with different dose of 30 keV energy N＋,which the doses were 0.5 × 1017ion/cm2-4.0 × 1017ion/cm2.we studied the Proline、MDA content and the activity of the POD、SOD of seedling leaves,The experiments set the blank control ck.The best treating dose was 3.5 × 1017 ion/cm2.At this dose conductivity,MDA content were low,the cell membrane was damaged small ; Proline、protein content and the activity of the POD、SOD of seedling leaves were high,N ＋ ion implantation could improved the resistance of maize.The result was useful for the N ＋ ion beam mutation breeding of maize.

关 键 词：玉米 N+离子束注入 诱变育种 生物学效应 

分 类 号：S513[农业科学—作物学]