海洋源放线菌Streptomyces sp.SCSIO 10428中吩嗪生物碱类抗生素的分离鉴定  

Isolation and elucidation of phenazine alkaloid antibiotics from the marine-derived actinomycete Streptomyces sp.SCSIO 10428

作　　者：吴正超[1,2] 郑六眷 陈耀龙[1,2] 李洁[1] 陈玉婵[3] 章卫民[3] 张长生[1] 李苏梅[1] 

机构地区：[1]中国科学院热带海洋生物资源与生态重点实验室中国科学院海洋微生物中心广东省海洋药物重点实验室中国科学院南海海洋研究所,广东广州510301 [2]中国科学院大学,北京100049 [3]广东省微生物研究所,广东广州510070

出　　处：《中国海洋药物》2014年第1期45-52,共8页Chinese Journal of Marine Drugs

基　　金：中国科学院知识创新工程重要方向项目(KZCX2-YW-JC202);国家自然科学基金(41006089;41106143;41106139)资助

摘　　要：目的对从广西北海斜阳岛海域沉积物中分离的1株海洋源放线茵Streptomyces sp．SCSIO 10428进行次级代谢产物及活性研究。方法对海洋源放线菌Streptomyces sp．SCSIO 10428的发酵产物进行有机溶剂萃取，利用硅胶、凝胶柱层析等手段纯化次级代谢产物，通过波谱数据分析及文献比较对化合物进行结构鉴定，对化合物进行了抗菌、卤虫致死以及抗氧化活性评价。结果从海洋源放线菌Streptomyces sp．SCSIO 10428发酵产物中分离得到3个生物碱类化合物，其结构分别鉴定为1-甲氧基吩嗪（1），1-羟基吩嗪（2），吩嗪-1-羧酸（3）；活性结果显示化合物1～3对白色念珠菌和苏云金芽孢杆菌具有较强的抑制作用，化合物2具有较强的卤虫致死活性和微弱的抗氧化活性。结论发现了1株能够产生3个不同吩嗪生物碱类抗生素的海洋源放线菌Streptomyces sp．SCSIO 10428。Objective To investigate the bioactive secondary metabolites from the marine-derived mycete Streptomyces sp. SCSIO 10428 isolated from a sediment sample from Xieyang island, Guangxi province. Methods The fermentation cultures of the actinomycete Streptornyces sp. 10428 were extracted with organic solvents and separated by column chromatography over silica actino- Beihai, SCSIO gel and Sephadex LH-20. The structures of the purified compounds were elucidated on the basis of spectroscop- ic data and comparisons with those reported in literatures. The compounds were evaluated for antibacterial, Artemia salina-lethal and antioxidative activities. Results Three phenazine alkaloids were ob- tained and elucidated as 1-methoxyphenazine （1）, 1-hydroxyphenazine （2） and phenazine-l-carboxylic acid （3） from the marine-derived actinomycete Streptomyces sp. SCSIO 10428. Compounds 1 -3 displayed significant antibacterial activities against Candida albicans ATCC 10281 and Bacillus thuringensis SCSIO BT01; compound 2 exhibited potent lethal activity toward A. salina and weak antioxidative ac- tivity. Conclusion This work provided a new marine-derived actinomycete Streptomyces sp. SCSIO 10428 which was capable of producing three phenazine alkaloid antibiotics.

关 键 词：海洋源放线菌 链霉菌属 SCSIO 10428 吩嗪 生物碱 生物活性 

分 类 号：R931[医药卫生—生药学]