小儿急性腹泻人博卡病毒364例分析  

Study on the prevalence of human bocavirus among 364 children with acute gastroenteritis

作　　者：王维[1] 林书祥[1] 黄敬孚[1] 徐晓华[1] 

机构地区：[1]天津市儿童医院天津市儿科研究所,天津300074

出　　处：《中国实用儿科杂志》2014年第1期48-51,共4页Chinese Journal of Practical Pediatrics

摘　　要：目的了解急性腹泻患儿中人类博卡病毒（HBoV）的感染情况。方法收集2008年8月至2009年7月在天津市儿童医院因消化道感染的急性腹泻门诊患儿粪便标本364份，提取病毒基因组DNA进行HBoVNP—1及NS1基因核酸片段的扩增，均成功扩增出目的片段者再以应用针对HBoV的核衣壳蛋白基因（VP1／VP2基因）的PCR引物进行HBoV基因片段检测。阳性标本作核酸序列测定，并与GeneBank中的已知序列进行序列比对和系统进化树分析。同时以PCR，RT—PCR方法检测粪便标本中诺如病毒、轮状病毒和腺病毒。结果PCR法检出HBoVDNA阳性率为3-3％（12／364）。阳性患儿中有41．7％（5／12）混合其他病毒感染，其中1例合并有2种病毒感染。感染患儿年龄分布从2个月到4岁，6个月至1岁感染HBoV患儿有9例，占HBoVDNA阳性患儿的75％。HBoVVP1／VP2基因片段与瑞典原型株sT2的核酸及氨基酸序列同源性分别为97％～99％及92％～99％，结论腹泻患儿的粪便中存在HBoV，为天津地区首次检测到。HBoV可能也是儿童急性腹泻中较为重要的一个感染原，且在1岁以内患儿中高发，有选一步研究的必要性。Objective To investigate the prevalence of human bocavirus （HBoV） among children with acute gastroenteritis （AGE） in Tianjin. Methods A total of 364 fecal samples from children with AGE in Tianjin Children＇ s Hospital were collected from August 2008 to July 2009. HBoV NP-1, NS 1 and VP gene fragments were detected by PCR. Positive PCR products were sequenced. The DNA and translated amino acid sequences were aligned with known HBoV sequences in GenBank and phylogenetic analysis was also done. Results Twelve （3.3%） fecal samples from children with AGE were tested positive for HBoV DNA. Multiple pathogens were detected in 5 （41.7 %） of the HBoV positive samples. The frequencies of HBoV positive samples in age groups 1 - 6 months, 7 - 12 months and 13 - 35 months were 4.8 %, 3.1% and 1.3 %, respectively. There were no HBoV positive samples detected in children 〈 1 month or 〉 4 years. HBoV positive rate was 7.6 % in spring （from March to May）, 1.1% in summer （from June to August）, 2.2 % in autumn （from September to November）, and 2.2 % in winter （from December to February）. The DNA and translated amino acid sequences of VP gene fragments of HBoV positive strains showed 97 % - 99 % and 92 % - 99 % identity with ST 2, respectively. Conclusion HBoV is found in fecal samples of 12 patients with AGE, and is more prevalent in infants younger than one year. Co-infections are frequent and clinically similar to HBoV infection alone.

关 键 词：急性腹泻 人博卡病毒 儿童 基因检测 序列分析 

分 类 号：R72[医药卫生—儿科]