乳腺癌转移瘤生物发光动物模型复制的实验研究  

Experimental study of bioluminescent breast cancer metastases nude mice model

作　　者：徐陆正 雷连娣[1] 

机构地区：[1]北京大学医药卫生分析中心分子影像学实验室,北京100191

出　　处：《中国现代医学杂志》2013年第32期36-40,共5页China Journal of Modern Medicine

摘　　要：目的复制尾静脉注射和腹腔注射接种的裸鼠乳腺癌转移瘤模型,应用生物发光技术监测肿瘤细胞在活体内的生长和转移。方法培养转染有荧光素酶基因的人乳腺癌细胞系MDA-MB-231-luc-D3-h2LN,分别通过尾静脉和腹腔接种到裸鼠体内,每周进行生物发光成像,最后解剖裸鼠体外观察转移灶。结果尾静脉接种模型肿瘤细胞在体内1周时有明显减少,之后在肺部定植并迅速增长,于接种后5周体外成像,确认肺部转移,其他部位未见肿瘤转移灶;腹腔接种模型的肿瘤细胞在腹腔形成多个转移灶,肿瘤细胞持续增殖,于接种后6周体外成像,发现壁层腹膜和脏层腹膜内多个转移灶,各脏器和腹腔以外部位未见肿瘤转移。结论建立了两种裸鼠转移瘤模型,并应用生物发光成像技术实现了深层部位肿瘤的追踪监测。[Objectives] To establish the intravenous and intraperitoneal injection of metastatic breast cancer nude mice models, and monitor the tumor cells growth and metastasis in-vivo using the bioluminescent imaging. [Methods] MDA-MB-231-1uc-D3-h2LN, the stable transfected luciferase gene human breast cancer cell line, is cultured and injected to nude mice by tail vein and vaccination, and then, the nude mice are bi oluminescent imaged weekly and ex-vivo imaged at last. [Results] From the tail vein vaccination group, tu mor cell number has an obvious decrease at the beginning, and then has a logarithmic growth during the fol lowing five weeks. All the tumor cells are at the lung while other parts have no metastatic tumor cells. From the intraperitoneal vaccination group, tumor cells have multiple metastases in the abdominal cavity, and tumor cell proliferation continues during the following six weeks. Multiple metastases have been found in the parietal peritoneum and visceral peritoneum, while no tumor metastasis has been found in the other organs and positions. [Conclusion] The two nude mice metastatic models have been built and the monitoring of deep parts tumors is realized.

关 键 词：生物发光 动物模型 肿瘤 

分 类 号：R737.9[医药卫生—肿瘤]