广林九号桉叶精油的抑菌活性研究  

Studies on Antimicrobial Activity of Essential Oil from Leaves of Eucalyptus urophylla × Eucalyptus grandis(Guanglin 9)

作　　者：田玉红[1] 周琪[1] 颇俊华 孟陆丽[1] 李梓[1] 

机构地区：[1]广西工学院生物与化学工程学院,广西柳州545006 [2]柳州华力家庭品业股份有限公司,广西柳州545006

出　　处：《湖北农业科学》2013年第21期5193-5196,共4页Hubei Agricultural Sciences

基　　金：广西自然科学基金资助项目(桂科自0991065)

摘　　要：研究了广林九号桉[Eucalyptus urophylla×Eucalyptus grandis(Guanglin 9)]叶精油的抑菌活性。利用气相色谱-质谱联用技术分析桉叶精油的化学成分,采用滤纸片法评价其抑菌活性,以平板稀释法测定了精油的最小抑菌浓度。广林九号桉叶精油的主要成分有:1,8-桉叶油素(40.844%)、α-蒎烯(24.221%)、α-松油醇(4.661%)、反式-松香芹醇(3.735%)、龙脑(2.762%)、乙酸α-松油脂(3.592%)和松香芹酮(1.978%)。广林九号桉叶精油对测试的菌种均有良好的抑菌活性,但对细菌和霉菌的抑菌效果有所差异。对金黄色葡萄球菌(Staphylococus aureus)、枯草芽孢杆菌(Bacillus subtilis)、大肠杆菌(Escherichia coli),黄曲霉(Aspergillus flavus)、黑曲霉(Aspergillus niger)和橘青霉(Penicillum citrinum)的最小抑菌浓度分别为10、20、20、10、10、5 mL/L。The antimicrobial activity of the essential oil from leaves of Eucalyptus urophylla×Eucalyptus grandis（Guanglin 9） was studied. The essential oil was analyzed by gas chromatography-mass spectrometry（GC-MS） method. The antimicrobial ac- tivity of oil was evaluated by using filter paper method and the minimum inhibitory concentration（MIC） was determined by plate dilution method. The main components of the essenti-al oil were 1,8-cineole（40.844%）, followed by α-pinene（24.221%）, α-terpineol（4.661%）, α-terpinyl acetate（3.592%）, borneol（2.762%）, trans-pinocarveol（3.735%） and pinocar- vone（1.978%）. The results indicated that the essential oil had excellent antimicrobial activity against tested microorganisms. The effects of essential oil on antibacterial and antifungal activities differed obviously. The minimum inhibitory concentrations（MICs） were 10,20,20,10,10 and 5 mL / L for Staphylococus aureus, Bacillus subtilis, Escherichia coli, Aspergillus flavus, Aspergillus niger and Penicillum citrinum, respectively.

关 键 词：广林九号桉[Eucalyptus Urophylla×Eucalyptus Grandis(Guanglin 9)]叶 精油 抑菌活性 最小抑菌浓度 

分 类 号：TQ654.2[化学工程—精细化工]