掺杂玉米茶叶的基因组DNA提取与PCR检测方法建立  

Extraction of Genomic DNA from Tea Doping with Maize and Establishment of PCR Detection

作　　者：余澍琼[1] 张吉红[1] 赵旭东 陈建国[1] 

机构地区：[1]宁波出入境检验检疫局,浙江宁波315012 [2]临沂市产品质量监督检验所,山东临沂276004

出　　处：《湖北农业科学》2013年第22期5615-5617,共3页Hubei Agricultural Sciences

基　　金：宁波检验检疫局科研项目(甬K04-2011)

摘　　要：根据玉米内源ZEIN基因建立了茶叶中掺杂玉米的普通PCR和实时荧光PCR检测方法。结果表明,标准CTAB法适合提取茶叶样品基因组DNA,实时荧光PCR的灵敏度能达到0.1%,普通PCR能达到1%,实时荧光PCR是普通PCR的10倍。两种方法都能达到检测要求。According to the endogenous gene ZEIN of maize, two PCR methods were established. The results showed that standard CTAB method was suitable for genomic DNA extraction of maize. Ordinary PCR had 1% detection sensitivity. The detection sensitivity of real-time PCR was 10 times more than that of ordinary PCR. Two methods can meet requirements for detecting maize in tea in future.

关 键 词：茶叶(Camellia sinensis) 玉米(Zea mays) 普通PCR 实时荧光PCR 检测方法 

分 类 号：Q78[生物学—分子生物学] S571.1[农业科学—茶叶生产加工]