依达拉奉对大鼠黑质神经元保护作用研究  

Study on Neuroprotective effects of edaravone on substantia nigra in rat model of parkinson's disease

作　　者：周宏智[1] 张元媛[1] 成亚琴[1] 陈鑫[1] 施建生[1] 

机构地区：[1]南通大学附属医院神经内科,江苏226001

出　　处：《交通医学》2013年第6期593-596,600,共5页Medical Journal of Communications

摘　　要：目的:探讨依达拉奉对6-羟基多巴胺(6-hydroxydopamine,6-OHDA)诱导的帕金森病大鼠模型的神经保护作用及其作用机制。方法:将实验动物分为神经生化学和组织学两组,各组分为⑴正常对照组6只;⑵生理盐水对照组6只;⑶依达拉奉组24只,依达拉奉又分为0.3mg/kg14d组6只,1mg/kg14d组6只,3 mg/kg14d组6只及3mg/kg28d组6只4个亚组。采用6-OHDA立体定位下注入SD大鼠左侧前脑内侧束制备帕金森病模型,观察依达拉奉对帕金森病模型大鼠的行为学改变、酪氨酸羟化酶阳性细胞数和谷胱甘肽的影响。结果:依达拉奉(3mg/kg14d)呈剂量依赖性减少APO诱发的6-OHDA大鼠帕金森病模型旋转次数(P<0.01),并能改善大鼠自发行为学变化。依达拉奉(3mg/kg14d.28d)阻止了6-OHDA导致的大鼠酪氨酸羟化酶阳性细胞数量和谷胱甘肽的减少(P<0.05)。结论:依达拉奉对6-OHDA诱导的帕金森病动物模型具有神经保护作用,依达拉奉改善6-OHDA损伤早期大鼠的行为学改变,抑制多巴胺神经元的脱失。Objective：To investigate the edaravone neuroprotective effect on the 6-OHDA-induced rat model of Parkinson＇s disease and its mechanism. Methods： The experimental animals were divided into two groups including the neurobiochemistry group and the histolology group, each of which was divided into three subgroups： the normal control group（n=6）, the normal saline （NS）group（n=6） and the edaravone group（n=24）, and the edaravone group was again divided into four subgroups including the 0.3mg/Kg 14d group（n=6）, the lmg/Kg 14d group（n=6）, the 3mg/Kg 14d group（n=6） and the 3mg/Kg 28d group（n=6）. The model of Parkinson＇ s disease was induced by injecting 6-OHDA into the left medial fore- brain bundle. The effects of edaravone on the behavior of the rat and the number of positive cells of tyrosine hydroxylase （TH） and glutathione （GSH）in substantia nigra were observed. Results： Edaravone （3mg/kg14d,28d） dose-dependently de- creased the rotation behaviors of rat models induced by apomorphine （P〈0.01）,ameliorated the spontaneous behavior, and prevented the decrease of the number of SN TH-positive cells and GSH induced by 6-OHDA（P〈0.05）. Conclusion： Edar- avone had neuroprotective effects on the 6-OHDA-induced PD animal model, improved 6-OHDA injury in early rat be- havioral change and inhibited depigmentation of DA neurons.

关 键 词：帕金森病 依达拉奉 6-羟基多巴胺 神经元 谷胱甘肽 免疫组织化学染色法 大鼠 

分 类 号：R742.5[医药卫生—神经病学与精神病学]