小细胞肺癌Nrf2与c-myc癌基因表达的相关性研究  

Correlation of NRF2 and C-MYC in samll cell lung cancer

作　　者：季俐俐[1] 王桂兰[1] 陈莉[1] 

机构地区：[1]南通大学医学院病理解剖学教研室,江苏226001

出　　处：《交通医学》2013年第6期607-610,共4页Medical Journal of Communications

摘　　要：目的:探讨小细胞肺癌c-myc癌基因的表达对Nrf2表达的影响,分析c-myc与Nrf2表达的相关性。方法:选用两株耐药性不同的肺小细胞癌肿瘤细胞株,小细胞肺癌药物敏感株H69及耐药株H69AR,Western-Blot方法比较Nrf2,c-myc在两株细胞间的表达差异;H69细胞通过Lipofectamine2000脂质体转染过表达c-myc,Western-Blot方法检测过表达c-myc前后Nrf2蛋白表达变化;H69AR细胞通过Lipofectamine2000脂质体转染c-myc siRNA,Western-Blot方法检测c-myc siRNA前后Nrf2的蛋白表达变化。结果:与H69细胞相比,H69AR细胞株中c-myc及Nrf2高表达;H69细胞c-myc转染后,Nrf2表达上调;H69AR细胞c-myc siRNA干扰后,Nrf2表达下调。结论:小细胞肺癌中Nrf2的表达与c-myc表达呈正相关。Objective：To determine the influence of oncogene c-myc over Nrf2 in small cell lung cancer, and to an- alyze the correlation between c-myc and Nrf2 expressions. Methods： Two lines of small-cell lung cancer cells were chosen in this study. One is drug sensitive H69 cell. The other is multidrug resistant H69AR cell. Western blot assays were uti- lized to compare the different expression levels of c-myc and Nrf2. Overexpression of c-myc by Lipofectamine 2000 trans- fection was performed in H69 cells, and Nrf2 protein expression was compared between the control and c-myc transfection groups. Down-regulation of c-myc by siRNA transfection was performed in H69AR cells, and Nrf2 protein expression was compared between the control and e-myc siRNA groups. Results： Compared with H69 cells, protein expression level of c- myc and Nrf2 was much higher in H69AR cell. In H69 tumor cells, Nrf2 was up-regulated after c-myc transfection. Mean- while, in H69AR tumor cells, Nrf2 was down-regulated after c-mye siRNA transfection. Conclusion： Nrf2 and c-myc is positively correlated in small cell lung cancer. To determine the influence of c-myc over Nrf2 in small cell lung cancer, and to analyse the correlation between c-myc and Nrf2 expressions.

关 键 词：小细胞肺癌 NRF2 C-MYC癌基因 Western-Blot检测法 

分 类 号：R734.2[医药卫生—肿瘤]