高产丙酮丁醇梭菌的诱变筛选及特性研究  

SCREENING AND CHARACTERIZATION OF A MUTANT OF Clostridium acetobutylicum WITH INCREASED BUTANOL TOLERANCE AND PRODUCTION

作　　者：高凯[1] 李云[1] 田沈[1] 杨秀山[1] 

机构地区：[1]首都师范大学生命科学学院,北京100048

出　　处：《太阳能学报》2014年第1期36-40,共5页Acta Energiae Solaris Sinica

基　　金：国家高技术研究发展(863)计划(2010AA101606)

摘　　要：使用亚硝基胍对实验室保藏的丙酮丁醇梭菌Clostridium acetobutylicum进行诱变,并结合外源添加丁醇的平板培养基对诱变菌株进行筛选,得到一株高产丁醇菌株。以60g/L葡萄糖为底物,37℃,发酵60h,该菌株丁醇及总溶剂产量分别比对照菌株提高了39.3%和48.4%。为了使溶剂产量最大化,针对不同底物浓度对溶剂产量的影响进行研究。结果表明,诱变菌株在利用80g/L葡萄糖为底物,发酵70h,得到最大总溶剂产量23.47g/L。As a gasoline substitute, butanol has advantages over traditional fuel ethanol in terms of energy density and hygroscopicity. However, solvent production appeared limited by butanol toxicity. The strain of Clostridium acetobutylicum was subjected to mutation by treatment with N-methyl-N＇-nitro-N-nitrosoguanidine （NTG）. Screening of mutants was done according to the individual resistance to butanol. A selected butanol-resistant mutant produced significantly higher solvent concentrations （48.4%） than the wild-type strain when 6% of glucose was employed as substrate. To maximize the solvent production by strain 6#, the effects of different concentrations of glucose on solvent production was further investigated. Results showed that strain 6 # is able to produce up to 23.47g/L solvents using 80g/L glucose after 70h fermentation.

关 键 词：丙酮丁醇梭菌 NTG诱变 丁醇耐受 高底物浓度 

分 类 号：TQ923[轻工技术与工程—发酵工程]